基于MHC的补肾复方对COH小鼠子宫内膜容受性调控机制研究

IVF-ET临床应用日益增多，子宫内膜容受性是IVF-ET成功与否的重要环节。妊娠类比同种异体移植，而MHC分子在同种异体移植中至关重要，其与妊娠密切相关，调控子宫内膜容受性。课题组认为“肾主生殖，为封藏之本”的理论与内膜容受性相关，鉴此，补肾安胎经典方“寿胎丸”能否通过调节MHC分子提高子宫内膜容受性？课题拟通过体内（COH模型小鼠）—体外（原代培养COH模型小鼠子宫内膜细胞）联合，采用液相芯片、Q-PCR、WB等方法，观察寿胎丸对COH模型小鼠种植窗期子宫内膜组织形态、内膜及外周血细胞MHC分子及其相关因子的影响，观察寿胎丸对子宫内膜细胞MHC分子及其相关因子的影响，分析寿胎丸能否通过调节MHC分子，从而提高子宫内膜容受性。从 MHC角度，在分子水平，揭示补肾复方改善COH子宫内膜容受性的调控机制。丰富中医“肾主生殖，为封藏之本”的客观化内涵，为IVF-ET中应用补肾复方提供依据。

The clinical application of In vitro fertilization-embryo transplantation (IVF-ET) is more and more popular. Endometrial receptivity is the key in IVF-ET success. Modern medicine takes pregnancy as allotransplantation, in which major histocompatibility complex(MHC) is essentially important. Since it controls and regulates the endometrial receptivity, it shows a close relationship with pregnancy. The research team realizes that TCM theory of “kidney governing reproduction as the dominator of essence storage” is related to endometrial receptivity. Thus, the purpose of the study is to explore if “Shou Tao Wan”(STW)—a classic formula of kidney-tonifying for miscarriage prevention, could increase endometrial receptivity by regulating MHC expression. The study is supposed to establish a pattern of COH mice model combined with primary cultured endometrial cell from COH mice model, with luminex, Q-PCR, Western blot, and so on, to detect influence of Shou Tai Wan on endometrial histomorphology at window stage for inplantation, MHC and its relevant factors expression in endometrium and peripheralblood cells. Meantime, the influence of STW on endometrial MHC and its relevant factors would be measured, to analyze that STW could increase endometrial receptivity by regulating MHC. We would try to reveal the molecular regulation mechanism of kidney-tonifying formula on improving endometrial receptivity from the aspect of MHC, therefore to enrich the TCM theory of “kidney governing reproductive as the dominator of essence storage” and provide evidences of integrating kidney-tonifying formula in IVF-ET.