仿生味觉自适应柔性纳米电极阵列构建研究
结题报告
批准号:
61901469
项目类别:
青年科学基金项目
资助金额:
24.5 万元
负责人:
张威
学科分类:
F0123.敏感电子学与传感器
结题年份:
2022
批准年份:
2019
项目状态:
已结题
项目参与者:
--
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中文摘要
Ⅲ型味觉受体细胞是仿生味觉信息传递的重要节点,在味觉刺激下能够发放动作电位并分泌五羟色胺(5-HT),但由于胞体较小、5-HT分泌量少且位点离散,现有方法无法完整而准确地在细胞层次上获取Ⅲ型味觉受体细胞的电生理和化学分泌特征,造成胞外重要关联响应的丢失,限制了味觉传导机理的研究。本项目提出一种仿生味觉自适应柔性纳米电极阵列,利用柔性基底与细胞膜微观匹配提高封接阻抗,增强味觉细胞与电极的有效耦合。通过石墨烯三维包裹纳米金属粒子修饰纳米电极阵列,增强电化学催化性能并提高灵敏度,实现对亚细胞尺度的nM量级多位点5-HT动态分泌的检测。记录并提取味觉细胞在味质刺激下胞外动作电位幅度、频率及5-HT动态释放等响应特征,建立一种多模态味觉细胞胞外电位/化学分泌耦联研究的新方法。这对揭示味觉细胞间、细胞与神经末梢的初级编码信息传导具有重要意义,在味觉修复,人工味觉和药物识别等领域有重要应用前景。
英文摘要
Type Ⅲ taste receptor cell is an important node for biomimetic gustatory information transmission. It could evoke action potential and serotonin (5-HT) secretion simultaneously under the tastant stimuli. However, technologies available could not analyze the electrophysiological and chemical secretory characteristics completely and accurately at the cell level, due to the small amount of 5-HT secretion and the discrete spot sites of the small taste cells. It is inclined to lead to correlation responses missing during important events, which limits the research of gustatory transmission mechanism. A self-adaptive flexible nano-electrode array for biomimetic gustation was developed in this project, which could enhance microscopic matching between flexible substrate and cell membrane and increase seal resistance. In order to realize multi-site 5-HT concentration detection as low as several nanomoles per liter at subcellular level, multisite nano-electrodes modified by nano metal particles 3D encapsulated with graphene were prepared for electrochemical catalytic performance and sensitivity improving. A novel approach was well established for coupling research on extracellular potential and chemical secretion of taste cells, which was utilized to record and extract the feature of extracellular action potential amplitude, frequency and dynamic release of 5-HT of taste cells under different concentrations of tastant stimulation. It is significant for revealing primary coding process and taste information transmission between cell-to-cell and cell-to-afferent fibers. It exhibits great potential for applying on gustation repair, artificial gustation and medicine recognition.
针对Ⅲ型味觉受体细胞胞体较小、5-HT分泌量少且位点离散,现有方法无法完整而准确地在细胞层次上获取Ⅲ型味觉受体细胞的电生理和化学分泌特征的问题,本项目提出并研究一种仿生味觉自适应柔性纳米电极阵列,利用柔性基底与细胞膜微观匹配提高封接阻抗,增强味觉细胞与电极的有效耦合。实现对亚细胞尺度的nM量级多位点5-HT动态分泌的检测。记录并提取味觉细胞在味质刺激下胞外动作电位幅度、频率及5-HT动态释放等响应特征,建立一种多模态味觉细胞胞外电位/化学分泌耦联研究的新方法。本项目中制备的6×6柔性电极传感阵列单元,薄膜厚度不大于500nm,5-HT浓度检测灵敏度达到nM量级。发表相关SCI论文7篇,申请发明专利5项,发明专利授权1项,培养研究生6名。本研究对揭示味觉细胞间、细胞与神经末梢的初级编码信息传导具有重要意义,在味觉修复,人工味觉和药物识别等领域有重要应用前景。
期刊论文列表
专著列表
科研奖励列表
会议论文列表
专利列表
The development of real-time digital PCR technology using an improved data classification method
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DOI:10.1016/j.bios.2021.113873
发表时间:2022-03-01
期刊:BIOSENSORS & BIOELECTRONICS
影响因子:12.6
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通讯作者:Zhou, Lianqun
The heparinase-linked differential time method allows detection of heparin potency in whole blood with high sensitivity and dynamic range.
肝素酶相关的微分时间法可以以高灵敏度和动态范围检测全血中的肝素效力。
DOI:10.1016/j.bios.2021.113856
发表时间:2021-11
期刊:Biosensors and Bioelectronics
影响因子:12.6
作者:Zheng Anran;Zhang Wei;Li Chao;Guo Zhen;Li Chuanyu;Zhang Changsong;Yao Jia;Zhang Zhiqi;Li Jinze;Zhao Shasha;Zhou Lianqun
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DOI:10.1063/5.0006374
发表时间:2020
期刊:Biomicrofluidics
影响因子:3.2
作者:Gao Xu;Li Jinze;Chuanyu Li;Zhiqi Zhang;Wei Zhang;Jia Yao;Ming Guan;Guo Zhen;Chuanyu Li;Lianqun Zhou
通讯作者:Lianqun Zhou
Heterogeneous modification of through-hole microwell chips for ultralow cross-contamination digital polymerase chain reaction
用于超低交叉污染数字聚合酶链式反应的通孔微孔芯片的异相修饰
DOI:10.1039/d0an00220h
发表时间:2020-04-21
期刊:ANALYST
影响因子:4.2
作者:Li, Jinze;Qiu, Yajun;Zhou, Lianqun
通讯作者:Zhou, Lianqun
Integrated microdevice with a windmill-like hole array for the clog-free, efficient, and self-mixing enrichment of circulating tumor cells.
具有风车状孔阵列的集成微装置,可实现循环肿瘤细胞的无堵塞、高效和自混合富集
DOI:10.1038/s41378-021-00346-y
发表时间:2022
期刊:Microsystems & nanoengineering
影响因子:7.9
作者:Li H;Li J;Zhang Z;Guo Z;Zhang C;Wang Z;Guo Q;Li C;Li C;Yao J;Zheng A;Xu J;Gao Q;Zhang W;Zhou L
通讯作者:Zhou L
国内基金
海外基金