S1P信号通路在花粉管生长中的功能研究

被子植物有性生殖过程需要借助花粉管将两个精细胞运送到胚囊中完成双受精。花粉管在雌蕊体内生长出现问题，会使精细胞运送到胚囊的效率下降，并最终导致植物育性的下降或是不育，从而造成农作物的减产。因此，研究花粉管体内生长的分子机制，有着重要的理论意义和实践价值。我们前期的研究结果证明鞘脂类物质鞘氨醇-1-磷酸（sphingosine-1-phosphate, S1P）通过调节膨压来调控花粉管生长。鉴于S1P在花粉管生长过程中的重要作用，我们将采用功能筛选和突变体分析两种方法来克隆拟南芥中的S1P受体，通过结合实验、以及结合后的生理生化功能分析，验证它们的配体—受体关系。分析突变体花粉管的功能，进一步阐明S1P受体介导的信号通路在花粉管生长调控过程中的作用机制。

Angiosperms evolved a tube like structure—pollen tube, to deliver the sperm cells to the ovule for double fertilization. When pollen tube growth is affected in pistil, it will have direct effects on reproduction efficiency and crop yield. Therefore, the research on molecular mechanisms of pollen tube growth has important theoretical significance and practical value. Our previous study proved sphingosine-1-phosphate (S1P) is a key regulator of turgor pressure in pollen tube. Due to its important roles in pollen tube growth, we will isolate the S1P receptor from Arabidopsis by functional screening and mutant screening. After binding assay, we will test its biochemical and physiological functions as S1P receptor to verify their roles as ligand and receptor. Furthermore, we will dissect the biological function of the S1P receptor by phenotype analysis of mutant pollen tubes. It will help us to better understand the role of S1P signaling in pollen tube growth.