母猪繁殖障碍问题严重影响养猪业的健康发展。有研究表明，应激影响雌性动物卵母细胞质量，导致繁殖力下降。研究猪卵母细胞对促肾上腺皮质激素（ACTH）的敏感性，对于了解应激及ACTH损伤卵母细胞发育能力的机制具有重要意义。然而，这方面的研究还鲜有报道。本项目将首先建立一个ACTH影响猪卵母细胞体外成熟但不影响小鼠卵母细胞成熟的模型。然后，观察调控ACTH功能的ACTH受体（MC2R）和受体辅助蛋白（MRAP）在两物种卵母细胞中的表达情况，并运用RNAi和基因过表达技术调控其表达，确定导致猪和小鼠卵母细胞间ACTH敏感性差异的原因。为了解应激及ACTH损伤猪卵母细胞发育能力的机制，以及生产实践中缓解应激对母猪繁殖力不利影响的相关药物研发，提供理论依据。

Reproductive disturbance severely affect the development of pork industry. Studies indicated that stress on female animals could impair oocyte quality leading to decreased fertility. Studies on the mechanisms for the sensitivity of pig oocytes to ACTH are of great importance for our understanding of the mechanisms by which stress and ACTH damage oocyte developmental potential. However, researches reported on this issue are few. In this project, we will first establish models in which ACTH impair in vitro maturation of pig oocytes while having no effects on that of mouse oocytes. Then, expression and activity of MC2R and MRAP, which regulate the function of ACTH, in oocytes of the two species will be compared by means of RNAi and gene overexpression techniques to determine the factors that cause ACTH sensitivity difference between pig and mouse oocytes. Data obtained will provide theoretical basis for our understanding of the mechanisms by which stress and ACTH impair pig oocyte maturation, and for the development of drugs that relieve the adverse effects of stress on female fertility in pig production practice.