肠道炎症疾病会给动物健康造成极大威胁。内质网应激能调节细胞内钙离子浓度和活性氧水平，并通过未折叠蛋白反应与炎症的发生发展紧密相连，可作为炎症性疾病新的治疗靶点。钩吻素子具有抗炎和调节免疫等活性。我们前期研究表明，钩吻素子可以缓解脂多糖诱导的巨噬细胞内活性氧水平升高，并可以通过调控炎症信号通路抑制炎症因子的过度释放。本项目提出钩吻素子抗炎作用有赖于内质网应激信号通路调控这一假设，以脂多糖诱导的体内肠道炎症模型和体外猪肠上皮细胞炎症模型，应用分子生物学的方法，研究钩吻素子对肠道炎症的影响，明确内质网应激是否参与钩吻素子抗炎过程，并利用基因沉默和克隆等技术构建内质网应激通路关键蛋白TXNIP和CHOP高低表达的猪肠上皮细胞模型，深入探讨内质网应激对钩吻素子抗炎作用的影响及其与炎症信号通路的相互关系。本项目将为钩吻素子临床应用提供科学依据，对促进动物健康、提高畜产品质量等方面具有重要意义。

Intestinal inflammatory disease is a great threat to animal health. Endoplasmic reticulum stress (ERS) can regulate intracellular calcium concentration and reactive oxygen level, which is closely linked to the development of inflammation through the folded protein response. Koumine has anti-inflammatory and immunoregulation activities. Our previous studies showed that koumine alleviated an increase of the level of reactive oxygen, and inhibited the excessive release of inflammatory cytokines though regulation of inflammatory signaling pathway in lipopolysaccharide-induced macrophage, indicating that ERS may be associated with koumine anti-inflammatory effects. A hypothesis which suggests that koumine anti-inflammatory effect depends on ERS regulation was proposed. In LPS-induced inflammation model in vivo and in vitro, this project investigates the effects of koumine on intestinal inflammation, whether ERS participates in koumine anti-inflammatory effects. By using the techniques of gene silencing and cloning to construct a cell model with high expression or low expression of key proteins (e.g. TXNIP and CHOP) in ERS pathway, the influence of ERS on the anti-inflammatory effects of koumine and the relationship between the inflammatory signaling pathway and ERS pathway were further discussed. This project will provide scientific basis for the clinical application of koumine, which is of great significance to promote animal healthy breeding and improve the quality of livestock products.