炎症在多个方面影响着人类健康。清热解毒类中草药多具抗炎活性，源于天然，是抗炎药物开发的重要方向。玉叶金花为壮族习用药材，具清热解毒、凉血解暑等功效，申请人前期研究发现其抗炎活性显著，但药效物质基础尚未能阐明。本项目以探究玉叶金花抗炎物质基础为目标，以中药代谢组学技术为依托，拟通过动物药理实验模型筛选玉叶金花抗炎活性部位，采用超高压液相色谱-四级杆飞行时间质谱技术分析活性部位及给药后血清和尿液中的代谢成分；结合主成分分析、正交偏最小二乘判别分析、变量重要性投影等化学计量学方法，对“活性部位-血清成分-代谢成分”的图谱进行表征，确定玉叶金花抗炎活性的代谢标识物；通过代谢标识物的变化规律，逆向追踪其关联化合物群，采用凝胶色谱、制备液相等技术进行分离纯化并解析其化学结构；建立体外炎症细胞模型进行抗炎活性筛选，阐明壮药玉叶金花抗炎药效物质及其作用机制；为岭南民族药物的发掘提供科学依据。

Inflammation affects the human health in many aspects. Most of Chinese herbal medicines, originating from nature and bearing the effect of clearing heat and detoxification, have anti-inflammatory activity. Research on it should be tendency of development of these anti-inflammatory medicines. Mussaenda pubescens Ait.f., a habitually used medicine among Zhuang tribe in China has various functions, such as clearing heat, detoxification, cooling blood, as well as clearing summer heat. The applicant has discovered its remarkable anti-inflammatory activity in the early stagee, however, the anti-inflammatory constituents haven’t been clarified yet. This project aims at studying the basics of the anti-inflammatory constituents and intends to sift the anti-inflammatory activity part through pharmacology experiment model, based on Chinese traditional medicine metabonomics technology. And the chemical components in the active parts of the Mussaenda pubescens Ait. f., together with the metabolic components in the blood serum and urine through dose toxicity in mice are to be analyzed adopting the method of UPLC-QTOFMS. And also the spectrums of active part-components of blood serum-metabolic components are to be characterized, through combining methods of chemometrics of PCA, PLS-DA, VIP, etc., so as to determine the metabolic marker of the anti-inflammatory activity in Mussaenda pubescens Ait.f. The correlative compounds group will be traced reversedly through the changing rule. The chemical structure is to be separated , purified and analyzed with methods, such as Sephadex LH-20, Prep-HPLC, etc. The extracorporeal inflammation cell model is to be established to sift the anti-inflammatory activity and the anti-inflammatory constituents and mechanism of functions of the Mussaenda pubescens are to be elucidated so as to provide the scientific basis to the exploration of the national medicine in Ling nan.