荧光共振能量转移介导的上转换荧光探针用于EBV-DNA的检测研究

EB病毒(Epstein-Barr virus, EBV)是最早被发现与人类肿瘤相关的病毒，及早和准确的检测引起该疾病的DNA序列对于EBV患者的诊断和治疗具有重要意义。针对目前临床中EBV-DNA检测技术要求高、误差大、易出现假阳性结果等问题，本项目提出以荧光共振能量转移原理(fluorescence resonance energy transfer, FRET)为介导，设计构筑基于以稀土上转换发光纳米粒子为能量给体，有机染料/金纳米为能量受体的DNA 纳米荧光探针，筛选高灵敏度、专一识别EBV-DNA的荧光探针，实现EBV-DNA的痕量检测。本研究将拓展上转换发光材料在生物方面的应用，丰富EBV-DNA的检测手段，对与DNA相关疾病诊断和治疗具有参考价值。

Epstein-barr virus (EBV) was found the earliest virus to be associated with human tumor, early and accurate detection of the DNA sequences of EBV is extremely important for the disease diagnosis and treatment. The current DNA detection methods in clinical application suffer from high cost and they are prone to false positive results. This project aims to design and construct novel up-conversion nanoprobes for highly sensitive EBV-DNA detection based on FRET (Fluorescence Resonance Energy Transfer). Up-conversion nanoparticles (UCNPs) and organic dyes /Au nanoparticle will act as the energy donor and the energy acceptor in the FRET system, respectively. This study will not only extend the applications of rare earth up-conversion nanophosphors in the biological applications, but also enrich EBV-DNA detection. Meanwhile, it has the reference value for DNA related disease diagnosis and treatment.