荧光共聚焦技术在生物体内Al3+离子检测中的应用

铝是人体非必需元素，但是由于该元素具有生物富集效应，给人体以及其它生物带来极大的危害。研究表明，老年痴呆症与Al3+在人体神经系统的富集有很大关系。因此，Al3+的检测/监测一直是研究的热点。在众多Al3+的检测方法中，荧光探针技术由于采用光信号作为输出信号，可实现损伤小、甚至无损伤地达到对生物体内目标物质的荧光成像检测，达到目标物质在生物体内的定性检测。但是，由于受到 Al3+配位性能的限制，Al3+荧光探针的设计及应用一直是学术界的难点。本项目在前期研究基础上，主要工作体现在以下三个方面：1）自主设计高选择性Al3+荧光探针；2）突破目前仅限于细胞内Al3+定性检测的现状，实现细胞体内Al3+的定量检测；3）利用复染技术，跟踪探针进入细胞体内的程度，实现细胞体内Al3+的定位检测。

Aluminum is a nonessential element in human body. Because of the biological enrichment effect of this element, great harm was brought to human body and other organisms. Therefore, many researchers focused on the detection/monitoring of Al3+. Among the methods for the detection of Al3+, fluorescence probe technique, using optical signal as output signal, has small damage or without damage for the detection of target substances in the body. However, due to the Al3+ coordination properties, the design and application of Al3+ fluorescent probe is always a difficult problem in academic circles. Based on our previous research, this project focuses on a breakthrough in the following three aspects: 1) the independent design of Al3+-selective fluorescence probe; 2) realize the quantitative detection of Al3+ in the living cells; 3) track the probe in the cells and locate the Al3+ in cells by counterstaining technology.