刺芹侧耳软腐病是近几年发生的新兴病害，已经在国内多个地方爆发，迄今尚无该病害病原菌致病机理的报道。本项目组在前期工作中，对国内发生的刺芹侧耳软腐病病原菌进行了分离、鉴定，发现了一个细菌新种，命名为北京泛菌；基因组测序结果表明，北京泛菌基因组中含有泛菌属等病原细菌关键致病因子编码基因hrp/hrc基因簇。因此，在前期研究工作的基础上，本研究拟绘制北京泛菌基因组精细图，通过基因组学分析，解析致病相关hrp/hrc基因簇组成与结构；利用比较基因组学手段，探索hrp/hrc基因簇的分子进化机制，以期解析致病分子机理。这是首次对刺芹侧耳软腐病病原菌-北京泛菌的关键致病基因及致病分子机理的研究，将为该病原菌的防治手段提供理论依据，具有重要意义。

Recently, soft rot disease occurred on Pleurotus eryngii is becoming an emerging disease in China. To date, there is no report on its pathogenic mechanism. In our previous study, pathogen to the soft rot disease of P. eryngii occurred in China was isolated and identified as Pantoea beijingensis sp. nov. Genomic sequencing analysis indicated that Panteoa beijingensis contained hrp/hrc gene cluster, which was reported to be the key pathogenic factor of the genus Pantoea and other bacterial pathogens. This research will study the key pathogenic hrp/hrc gene cluster by genomic analysis. Furthermore, molecular evolutionary relationship within the pathogenic Pantoea spp. based on hrp/hrc gene cluster will be illustrated by comparative genomic analysis. This is the first study on the molecular mechanisms of Pantoea-induced soft rot disease of P. eryngii. The result of this research will lay significant foundation for the purpose of controlling this desease in practices.