淋巴结是宫颈癌转移的第一站，前哨淋巴结微环境是否参与促进肿瘤转移尚不清楚。申请者所在课题组利用激光捕获和表达谱芯片发现Semaphorin 4C(Sema4C)在人宫颈癌相关淋巴管内皮细胞（LEC）中高表达，并意外发现其具有分泌型，在人的血清中被检测到和淋巴结转移正相关。申请者初步实验发现Sema4C在宫颈癌淋巴结转移的淋巴管内皮细胞中高表达，因此，本项目拟进一步明确Sema4C在淋巴结转移前微环境中发挥的作用，利用裸鼠建立动物模型，局部或全身使用蛋白Sema4C，模拟肿瘤转移前淋巴结内淋巴管变化，检测淋巴管密度和粘附能力的改变，探讨Sema4C建立宫颈癌淋巴结转移前微环境的机制。旨在揭示宫颈癌淋巴结转移前前哨淋巴结一系列分子改变，为术中淋巴结显影和临床治疗提供新的选择。

Lymph nodes are initial sites of Cervical cancer metastasis, yet whether the lymph node microenvironment actively promotes tumor metastasis remains unknown. In preliminary studies, we found that Cervical carcinoma associated LECs（lymphatic endothelial cells）highly express and secrete Semaphorin 4C(Sema4C), which could promote LECs proliferation, movement and tube sprouting. Besides, secrete Sema4C could be detected in the serum of patient by ELISA and had a positive correlation with lymph node metastasis. Yet applicant found that highly expression of Sema4C in metastatic lymph node LECs and therefore, this project intends to further clarify the role of Sema4C plays in the metastasis microenvironment in draining lymph nodes, by establishing nude mice animal model, using protein Sema4C local or systemic, imitating pre-metastasis niche of sentinel lymph nodes and detecting changes of lymphatic vessel density and adhesion ability, to explore the mechanism of Sema4C establishing pre-metastasis niche of sentinel lymph nodes in cervical carcinoma. The aim of this study was to reveal a series of molecular changes of sentinel lymph node before lymph node metastasis in patients with cervical cancer,.to provide a new choice for the Intraoperative lymph node imaging and clinical treatment.