番茄根际K策略细菌群落的装配及其抑病机制研究

土壤微生物生存策略与其生态功能具有密切联系。本实验室前期已通过连续14季田间试验构建了番茄抑青枯病土壤，并发现土壤细菌群落生存策略与番茄植株抑病能力显著相关，且抑病型番茄根际细菌群落会在青枯菌入侵时向K策略（高抵抗力）演替。但抑病型根际细菌群落生存策略的生育期演变特征以及病原菌入侵时根际生存策略的权衡机制还未系统研究，K策略根际细菌群落的抑病机理也未得到解析。本项目以长期田间试验定位点为依托，基于已人为调控形成的抑病型土壤，以根际细菌群落为研究对象，解析抑病型根际细菌群落生存策略随植物生长的变化规律及其功能特征；揭示病原菌入侵时植物驱动抑病型根际细菌群落向K策略的演替机制；同时，利用培养组技术结合功能特征检测，评估可培养细菌的生存策略，构建不同生存策略菌群组合，解密K策略细菌群落抵御病原菌入侵机理。最终为抑病型根际微生物的调控提供新的理论指导。

Life strategy of soil microbiome can reveal its ecological functions. Through a 14-season consecutive field experiment, we found life strategies of the soil bacterial community was significantly related to bacterial wilt disease control ability, and bacterial community of suppressive rhizosphere changed to K strategy（higher resistance） when pathogen invasion. However, there is not systematically study about the life strategies traits of suppressive rhizosphere microorganisms along with tomato growth period and the trade-off mechanism of rhizosphere life strategies during pathogen invasion. Moreover, the suppress mechanism of K strategy rhizosphere microbial community has also not been uncovered. This project is based on suppressive soil of the long-term field experiment, to analyze changes of life strategies and functional traits of the suppressive rhizosphere bacterial community along with plant growth period; to reveal the trade-off mechanism of suppressive rhizosphere microorganisms when pathogens invasion; meanwhile, we would combine culturomics and functional traits to quantify the life strategies of microorganisms, and further construct artificial consortia of gradient life strategies to decipher the suppress mechanism of K strategy rhizosphere bacterial communities when pathogen invasion. Therefore, the achievement will provide new theoretical guidance for the regulation and construction of suppressive rhizosphere.