拟南芥中DET1通过稳定PIFs抑制暗中光形态建成的机制研究

拟南芥光形态建成抑制因子De-etiolated 1 (DET1)或Phytochrome-Interacting Factors （PIFs）突变后，植物幼苗在暗中都可以完成光形态建成，它们的协同作用机制还不清楚。我们的初步研究表明，酵母双杂交中DET1可以和PIFs家族转录因子相互作用。暗中生长的拟南芥幼苗中，DET1可能正调控PIFs转录因子的蛋白含量。本项目的目的是解析DET1通过与PIFs蛋白相互作用抑制光形态建成的机制，其研究内容包括：(1) 验证DET1与PIFs 蛋白之间的相互作用；(2) DET1对PIFs蛋白稳定性的调节；(3) DET1与PIFs的遗传学相互作用；(4) DET1对PIFs靶基因的调节；（5）其他COP/DET/FUS因子与PIFs的相互作用。通过这些方面的探索，植物暗中光形态建成抑制的作用机制将更多地展现出来，植物中的光信号转导通路将会更为明晰。

Arabidopsis seedlings exhibit photomorphogenic development even in darkness, if the repressor of photomorphogenesis De-etiolated 1 (DET1) or Phytochrome-Interacting Factors （PIFs） are disrupted. However, the regulation mechanism on how these factors work in concert is unknown. In our preliminary test, yeast two-hybrid assay results indicated that DET1 may directly interact with a group of transcription factors PIFs. In the dark grown Arabidopsis, DET1 may positively regulate the accumulation of PIFs proteins. The Objective of this project is to analyze the mechanism of how DET1 interacts with PIFs to repress photomorphogenesis in darkness. The project will include: (1) Analysis of the interactions between DET1 and PIFs proteins via multiple strategies; (2) Analysis on how DET1 regulates the stability of PIFs; (3) Analysis of the genetic interactions between DET1 and PIFs; (4) Analysis on how DET1 regulates the PIFs targeting genes. （5）Analysis of interactions between other COP/DET/FUS factors and PIFs. After these tests, more mechanisms of the photomormogenesis repression in darkness will be shown, and light signal transduction pathways in plant will be understood better.