潮热是更年期综合征的典型症状。贯叶连翘能明显改善更年期代谢紊乱所致的潮热症状。虽然鲜有从代谢紊乱角度来探索贯叶连翘改善更年期潮热机制的报道，但本课题组前期发现更年期潮热与“精氨酸-蛋氨酸-色氨酸（AMT）”代谢通路有显著关联性，而贯叶连翘能够明显逆转该通路中代谢物的紊乱水平。因此本项目拟进一步从代谢通路中的代谢物和相关代谢酶角度切入，基于靶向代谢组学，以液质联用分析和多肽同位素标记技术定量测定去卵巢更年期模型大鼠造模前后及贯叶连翘干预前后AMT通路代谢物水平及相关代谢酶含量，构建“贯叶连翘-AMT代谢通路（代谢物-代谢酶）-更年期潮热”调控体系，验证贯叶连翘改善更年期潮热与代谢通路“紊乱-平衡”的机制，并为临床预测、诊断更年期代谢紊乱疾病提供新思路。

Hot flash is the typical symptoms of menopause syndrome. Hypericum perforatum L. (St John`s Wort) has been used to release menopausal syndromes for centuries. Results from the comparison of metabolite levels of OVX rats before and after the administration of different doses of Hypericum perforatum show that Hypericum perforatum can not only can reverse the disordered changes of multiple biomarkers of menopausal syndrome but also demonstrate therapeutic functions that are related to relevant enzyme activities and the saturation of relevant receptors which focus on ‘Arginine - Methionine - Tryptophan (AMT)’metabolic pathways. Therefore, this project aimed to explore the relationship between metabolic pathway metabolites and related metabolic enzymes, based on targeted metabolomics via HPLC-MS and isobaric tags absolute quantitation technologies among control, ovariectomized and treatment groups. By screening, identification, and quantification of the differential regulatory metabolites, a ‘Hypericum-AMT metabolic pathways (metabolites - enzymes) - menopausal hot flashes" regulating system will be established to verify the mechanisms of Hypericum perforatum L. in the treatment of menopausal hot flashes. Finally, the mechanism of‘Menopausal disorder - balance’will open the fields for menopausal clinical diagnosis.