单碱基多形态研究中，建立超高灵敏的生物分子识别与简便的检测方法是揭示生命体系微观分子机制不可或缺的手段。而以往的研究忽略了DNA强大的复制能力，也没有克服识别探针和信号探针分离设计的信号内耗问题。本项目拟在基因分子理论学、临床诊断技术及基因突变检测方法学等系统性研究评价的基础上，以急性白血病基因点突变为研究模型，借助识别探针和信号探针一体化设计技术，同时鉴于G-四链体-氯化血红素（Hemin）DNA 酶在传感器设计中所表现出来的独特优势，发展利用聚合酶、剪切酶、连接酶等DNA修饰酶的生物特异识别性质开展区分基因突变的分析新原理，设计DNA酶催化显色和酶催化电流响应等信号高效转换的新机理，建立环保、快速、可靠、低成本、高通量的基因分析新方法，推动其在急性白血病和肺癌等各类基因突变的疾病早期诊断中的应用，研究成果具有广泛的理论意义与应用前景。

Basing on the research of single polymorphic nucleotides, the untrasensitive biomolecular recognition detection and convenient method is important to reveal the molecular mechanisms of the microscopic life system.At last,The studies were not only ignore the powerful repplicate ability of DNA, but also overcome the problem of signal friction. The project intends to evaluate on the basis of a systematic study of genetic and molecular theory, clinical diagnostics and gene mutation detection methodology. And the research model of the project is acute leukemia gene mutation, at the same time, the G-quadruplex-Hemin DNA enzyme sensor design is developed that G-quadruplex-Hemin possess the unique advantages. And for good detection limit,a lot sorts of protease are used for signal amplification, including polymerase, ligase and exonuclease,etc. It execute that the organisms specifically identify the nature of the principle of distinction to carry out analysis of new mutations. The designs of chromogenic enzymatic DNA and the current response to other signals enzymatic conversion of new mechanism are constrcuted, which hold a rapid, reliable, low-cost, high-throughput genetic analysis of new methods that its application of research results in mutation detection and early diagnosis of acute leukemia in a wide range of theoretical significance and application prospects.