稻米重金属污染问题已引起国民的高度重视，而稻田土壤中重金属砷的存在形态直接影响水稻对砷的吸收和富集，所以开展稻田土壤中砷形态的转化研究对控制水稻砷的吸收、减少稻米砷污染具有重要意义。本项目主要研究单克隆砷氧化菌CRS003对砷污染稻田土壤中砷的形态转化的作用，包括详细探讨砷氧化菌接种比例、有机碳氮源种类及含量、搅动方式、接种周期及土壤灭菌等因子对该菌株在稻田土壤中的定殖及砷形态转化效率的影响。同时，详细研究砷污染水稻土壤中CRS003对砷形态迁移特性的影响及水稻对土壤中不同形态砷的摄取和富集。通过上述两方面的研究，探索利用砷氧化菌CRS003调控砷污染稻田土壤中砷的形态进而达到控制水稻对砷的摄取和富集的可能性，为砷污染区域水稻的砷污染控制提供理论依据和技术支撑。

The heavy metal pollution of rice grains has caused the great national attention, moreover, the species of arsenic in the paddy soil directly affect the intake and accumulation of arsenic in the rice plant. Therefore, it is very important to study the species transformation of arsenic in paddy soil for controlling the uptake of arsenic in the rice plant and reducing the arsenic pollution of rice grains. In this study, the transformation of arsenic species in paddy soil in the presence of monoclonal arsenite-oxidizing bacteria CRS003 will be studied. Especially, the inoculation percent of CRS003, organic carbon and nitrogen concentration and types, ways to stir, and inoculation period on the colonization of CRS003 and its transformation efficiency for arsenic species in paddy soil will be studied in detail. At the same time, the migration characteristics of different arsenic in paddy soil and intake/accumulation of different arsenic in the rice plant will also be studied in detail in the presence of CRS003. Based on above experimental results, we try to investigate the possibility of controlling the species transformation of arsenic in paddy soil by using arsenite-oxidizing bacteria CRS003 in order to finally control the intake and accumulation of arsenic in the rice plant. The results of this study will provide a theoretical basis and technical support for controlling the intake of arsenic in the rice plant, which growing in arsenic pollution area.