丙二烯氧化物合成酶（AOS）是茉莉酸合成途径中的关键酶，害虫取食能够诱导AOS基因的表达，从而间接调控植物的抗虫反应。阐明水稻AOS基因对害虫取食的应答规律及机制，对揭示水稻-害虫互作及协同进化的内在机理具有重要意义。本研究拟首先克隆水稻4个AOS基因的启动子，将启动子与报告基因融合，明确AOS基因启动子对咀嚼式、刺吸式口器害虫取食的应答模式，并通过5'-end缺失、凝胶阻滞实验、定点突变等方法，鉴定AOS基因启动子中对刺吸式、咀嚼式口器害虫取食产生应答的顺式元件，从启动子结构层面解析AOS基因对害虫取食的应答机制，揭示水稻被不同口器害虫取食后产生特异防御反应的内在原因。

Allene oxide synthases (AOS) is a key enzyme in the pathway of synthesis of jasmonic acid. The expression level of AOS is regulated by insect feeding, and this mediates regulation of insect-responsive defense. Illuminating the pattern and mechanisim of OsAOSs responding to insect attack will afford new molecular evidence for shedding light on the mechanisms of interactions and co-evolution between insects and plants. In this project, we will clone the promoters of four rice AOS and fuse the promoter with reporter gene, analyze the responding pattern of AOS promoters to chewing and phelom-sucking insects, identify chewing and phelom-sucking insect feeding-responding cis-elements in promoter regions, try to explain the mechanisim of OsAOS's promoter responding to insect attack on the basis of the structure of promoter, discover the intrinsic mechanisms of specific defense of plants to different kinds of insects.