PLLA/PDLA三维支架与滑膜间充质干细胞/软骨细胞构建颞下颌关节盘的研究

目前，对于颞下颌关节盘破裂与穿孔尚无理想的治疗方法，亦无利用滑膜间充质干细胞与软骨细胞共同构建颞下颌关节盘的报道。所以，本研究选用这两种细胞作为种子细胞。以I型胶原（collagen type I, COL-I）、左旋聚乳酸（poly-L-lactic acid, PLLA）、消旋聚乳酸（poly-D-lactic acid, PDLA）为原料，通过电纺丝法制备海绵状P(L/D)LA 96/4和膜状P(L/D)LA 70/30两种材料，将二者合为上下两层P(L/D)LA材料，COL-I与双层P(L/D)LA复合成COL-I/P(L/D)LA支架。滑膜间充质干细胞/软骨细胞三维培养于支架中，特定的细胞因子诱导细胞支架复合物，然后植入颞下颌关节盘穿孔的动物体内，了解其在颞下颌关节盘组织工程中的作用。本研究创新之处在于探讨这两种细胞混合培养后向纤维软骨分化，选用的支架在结构上更模拟颞下颌关节盘。

Up to now, there is no good treatment for the perforated temporomandibular joint (TMJ) disc, and there is also no article that reported the application of co-cultured synovial mesenchymal stem cells (SMSCs)/articular chondrocytes (ACs) for the tissue engineering of TMJ disc. In this study, SMTCs and ACs were applied as seeding cells, and collagen type I (COL-I) and poly-L-lactic acid (PLLA) as well as poly-D-lactic acid (PDLA) as scaffold. Through the method of electronic spinning, the spongy-like P(L/D)LA 96/4 and membrane-like P(L/D)LA 70/30 were fabricated and sutured together, and then COL-I were added into the P(L/D)LA scaffold. The SMSCs/ACs were cultured onto the P(L/D)LA scaffold and induced by cytokines, and the cells/scaffold constructs were transplanted into the perforated TMJ disc rabbits. Then the constructs were examined to explore its physical and the molecular characterstics. The novelty of this study might be that the SMSCs and ACs were co-cultured as feeding cells in the TMJ disc tissue engineering.