生物功能性外基质3D打印支架与共培养修复髁突软骨缺损及机制的研究

由于软骨独特结构和特殊性质，软骨缺损或退行性病变很难自愈。组织工程化软骨修复为其提供了新的思路。本研究，拟通过3D打印PCL-HA支架材料，将转染TGFβ3的脂肪间充质干细胞接种于PCL-HA支架材料中，使其产生软骨细胞外基质分布于PCL-HA支架中，之后将脂肪间充质干细胞脱去，获得富软骨细胞外基质的ECM-PCL-HA支架材料。将前期研究中使用的滑膜间充质干细胞与关节软骨细胞混合培养于ECM-PCL-HA支架材料中，用于修复髁突软骨缺损，评估修复效果。本研究的创新之处在于利用转染TGFβ3的脂肪间充质干细胞接种于3D打印的PCL-HA中，在体外利用转染后的脂肪间充质干细胞分泌产生软骨细胞外基质分布于PCL-HA支架中，然后将细胞脱去，获得无病毒负载的ECM-PCL-HA支架。最终为细胞外基质支架的临床应用提供理论依据。

Due to the unique structure and special properties of cartilage, once the cartilage defects or degenerative diseases are difficult to heal，Tissue engineered cartilage has opened up a new idea for this. By using 3D-printed PCL-HA scaffold, this study intended to inoculate the adipose mesenchymal stem cells of transfection TGFβ3 into the PCL scaffold to produce cartilage extracellular matrix which would spread in the PCL-HA scaffold which 3D-printed. Later the adipose mesenchymal stem cells were decellularized and the ECM-PCL-HA scaffold in cartilage extracellular matrix were obtained. The synovial-derived mesenchymal stem cells used in previous studies and the articular chondrocytes were co-cultured in the ECM-PCL-HA stent rich in cartilage extracellular matrix for repairing condylar cartilage defects. The restoration outcomes were evaluated. Innovative step of the study consisted in inoculating the adipose mesenchymal stem cells of transfection TGFβ3 into the 3D-printed PCL-HA scaffold. So far, there haven’t been relevant reports both at home and abroad on the practices that the transfected adipose mesenchymal stem cells are used in vitro to generate cartilage extracellular matrix to spread in PCL-HA scaffold and then the cells are sloughed to get the virus-free ECM-PCL-HA scaffold rich in cartilage extracellular matrix. By doing this study, there can be provided a theoretical basis for the clinical application of extracellular matrix scaffold.