环状RNA(circRNA)是新发现的一类调控肿瘤发病的重要非编码RNA分子，然而对多发性骨髓瘤（MM）的调控机制研究仍为空白。我们前期已筛选出特异性高表达的hsa_circ_0077306和RRAGD呈正相关，二者拥有共同的miR-320结合位点，可进一步调控下游mTOR通路的功能。据此，本课题将从细胞、分子、临床和动物模型四个层面，采用芯片技术、生物信息学、CHIP、Q-RT-PCR、Western Blot、RIP及EMSA等技术探讨和阐述环状RNA hsa_circ_0077306通过调控miR-320/RRAGD/mTOR轴介导多发性骨髓瘤的发病机制，从而对认识MM发病和circRNA等关键科学问题上提出新发现，为推进精准个体化治疗提供新靶点。

Circular RNAs (circRNAs) are newly discovered and emerging as an important class of regulatory transcripts that are implicated in a variety of biological functions in turmor, which has become the area of concern in ncRNA field. However, there is still unknown the mechanism of circRNA in multiple myeloma (MM). Our previous work has been identified the circRNA and mRNA expression pattern and found the distinct circRNA hsa_circ_0077306 and mRNA RRAGD expression in positive correlation pattern in MM. The bioinformatic predict that hsa_circ_0077306 functions as a competitive endogenous RNA to regulate RRAGD expression by sponging miR-320 and to regulate mTOR pathway activity. Therefore, we intend to explore deep regulation function mechanism of the hsa_circ_0077306 mediated miR-320/RRAGD/mTOR axis in vitro and in vivo from cell, molecular, clincial and animal model different level by ceRNA chip, bioinformatic, CHIP, Q-RT-PCR, Western Blot, RIP, and EMSA assay.This study will to demonstrate the molecular mechanism and thus lay the foundation for further research and explore promising treatment regimens.