临床上椎间盘源性坐骨神经疼痛由于致痛原因的多样化，导致了其疼痛神经学机制的复杂化。尽管越来越多的研究证实脊髓胶质细胞参与多种慢性疼痛模型痛的诱导和维持，然而其在不同刺激模式诱致的椎间盘源性坐骨神经疼痛中的作用和机制还不清楚。本研究拟在以往研究的基础上，从神经学的角度出发，采用行为药理学、免疫组织化学、免疫荧光、激光共聚焦扫描成像及分子生物学技术，深入探讨不同刺激模式诱致的椎间盘源性坐骨神经痛中脊髓胶质细胞的作用和机制，以期为临床椎间盘源性坐骨神经疼痛的个性化治疗提供新的思路和理论依据。

Discogenic sciatica induced by multiply causes results in complex neuromechanisms of pain. More and more studies have confirmed that spinal glial cells are involved in pain induction and maintainance in various kinds of pain models. However, whether spinal glial cells are also involved in sciatica induced by different styles of lumbar disc herniaton has not been confirmed. On the basis of our previous studies, this research aims at investigating the mechanisms of spinal glial cells (microglia and astrocyte) in sciatica induced by different stimulating styles from various methods including behavior pharmacology,immunohistochemistry,immunofluorescence,laser confocal scanning imaging, molecular biology and so on. This study aims at providing an important strategy for the individual treatment of discogenic sciatica in clinic.