我国海洋生物资源丰富，海洋生态环境的特殊性，造就了海洋生物能产生与陆生动植物更加独特的次生代谢天然产物，而快速高效的提取、分离和活性鉴定这些次生代谢产物是海洋天然活性物质筛选的瓶颈。本项目拟集成加速溶剂萃取（ASE）、高速逆流色谱（HSCCC）和二苯基三硝基苯肼（DPPH）开发设计ASE-HSCCC-DPPH联用装置，充分发挥ASE快速高效样品提取、HSCCC高效分离、纯化、制备的特点及DPPH作为抗氧化活性筛选通用方法的优势，并将其应用于海洋天然活性物质研究，为海洋天然产物高通量筛选提供理论和实践可行性。这一联用技术的实现对于推动海洋生物活性物质开发，发现海洋天然活性成分具有十分重要的意义。

China's marine resources are rich. Due to the particularity of the marine environment, marine organisms can produce more unique secondary metabolites natural products compared with terrestrial animal and plant. It has become a bottle-neck for screening and isolation of active substances from marine natural products. An on-line method based upon accelerated solvent extraction (ASE), high-speed countercurrent chromatography (HSCCC) coupled with 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) was developed for continuous isolation of active substances from marine natural products. Accelerated solvent extraction (ASE) has several advantages over traditional solvent extraction methods, including shorter extraction time, lower solvent consumption, higher extraction yields, high reproducibility and less extraction discrimination. HSCCC is a simple, fast and non-complex solvent system technique to isolate and purify compounds from natural plant; The DPPH oxidative assay adopted in the paper is used worldwide in the quantification of radical-scavenging capacity. It was strongly believed that the proposed method would be promising for separation, purification and activity identification of useful substances from marine natural products.