2013年以来我国反复发生较大规模的由柯萨奇病毒A组6型（CV-A6）引起的手足口病（HFMD）暴发，CV-A6已经成为我国HFMD病原谱的重要病原体，并且导致较多重症HFMD病例。本研究拟解决CV-A6基因变异与其致病性（毒力）之间的关系这个HFMD防控和基础研究中的关键科学问题。研究中拟综合应用生物信息学方法，阐明我国CV-A6的遗传进化规律，完善对其基因特点、基因分型、传播规律的认识，追踪病毒抗原决定簇变异情况；发现一些重要的温度敏感性相关位点，探讨CV-A6在近年来成为我国HFMD主要病原体的原因。其研究结果将为我国肠道病毒病的预防控制提供重要的基础数据，对制定有效的HFMD预防和控制措施具有较重要的公共卫生意义和实际应用价值。

Repeated larger scale hand foot mouth disease (HFMD) outbreaks caused by coxsackievirus A6 (CV-A6) occurred in China since 2013, and CV-A6 has become an important pathogen in HFMD pathogen spectrum, and lead to lots of severe HFMD cases during the transmission. This study will resolve the scientific problem of the relationship between CV-A6 genetic mutation and its pathogenicity (virulence), which is also a key scientific issue in the HFMD prevention and control and basic research. Comprehensive application of bioinformatics methods will be performed in this study in order to expound the genetic evolution law of CV-A6 circulated in China, and to improve the understanding of its genetic characteristics, genotyping and transmission pattern, and to track the mutations of epitope determinants of CV-A6. This study will find some important temperature sensitivity determinant sites, and to explore the reasons of CV-A6 becoming the main pathogen of HFMD in recent years in China. The results of the study will provide important basic data for the prevention and control of enteroviral diseases in our country, and has important public health significance and practical application value to the development of effective HFMD prevention and control measures.