肝癌发病隐匿，治疗效果及预后较差，早期诊断及有效的治疗成为关键。MRI分辨率高、图像对比度良好，可以从分子水平对靶细胞进行无创监测。超顺磁性氧化铁纳米粒子(SPION)作为一种新型的MRI造影剂，具有良好的生物特性，可以和聚和物胶束联合应用于抗癌药物传递和靶向配体相结合，可实现对肿瘤的靶向诊断和治疗。前期我们合成壳聚糖-油酸聚合物胶束，胶束核内包载SPION，可提高SPION的稳定性和生物相容性，表面形成的核壳结构可进一步搭载药物或靶向配体。研究表明表皮生长因子受体(EGFR)在肝癌中高表达，与肝癌的形成、发生和发展有密切的关系，是潜在的重要靶点。因此，本课题拟制备靶向EGFR同时包载抗癌药物的多功能纳米胶束，通过体外细胞实验及肝癌动物模型，评估共载SPION和阿霉素的EGFR靶向胶束对肝癌具有特异性磁共振诊断的同时，对肝癌是否有明显的抗肿瘤作用，为肝癌MRI诊断及治疗提供新方法。

Hidden occurrence of Hepatocellular carcinoma (HCC),treatment and prognosis are poor, early diagnosis and effective treatment become the key. Targeting cells can be monitored non-invasively by Magnetic resonance imaging(MRI) with high resolution and good image contrast. As a new MRI contrast agent with good biological characteristics, superparamagnetic iron oxide nanoparticles (SPION) can be combined with polymeric micelles for anticancer drug delivery and targeting ligands for targeted diagnosis and therapy of tumors. Chitosan-oleyl micelles encapsulating SPIONs with good biocompatibility and stability are prepared by our laboratory, and we can modify drugs and targeting ligands on their surface. Epidermal growth factor receptor (EGFR) as a potential important therapeutic target is highly expressed in HCC and closely related to the morbidity and mortality of HCC.In conclusion, this topic intends to prepare targeting EGFR containing multifunctional micelles with anti-cancer drugs and through in vitro cell experiments and Hepatocellular Carcinoma animal model evaluate that whether EGFR targeting micelles containing SPION and doxorubicin with specific magnetic resonance diagnosis has significant anti-tumor effects of Hepatocellular Carcinoma at the same time, and provide a new method for MRI diagnosis and treatment of Hepatocellular Carcinoma.