microRNA是一类非编码的小分子RNA，广泛参与了细胞的分化、发育及各种临床疾病的发生发展。本课题我们首先对反复自然流产患者和正常妊娠妇女的绒毛和蜕膜组织进行microRNA 芯片分析，通过差异microRNA筛选及功能预测,我们发现mir-184在反复自然流产患者的绒毛和蜕膜组织中均显著高表达，且其靶基因分析显示mir-184与细胞发育、分化、免疫应答等功能密切相关。因此我们推测，mir-184在反复自然流产中可能起到关键调控作用。本研究拟在已有预实验的基础上，通过细胞生物学和分子生物学实验，进一步探讨mir-184对胎盘滋养细胞生物学行为的影响；并阐明mir-184引起的这些生物学功能改变的机制，即mir-184通过调控那些基因的表达来引起滋养细胞生物学行为变化。通过对以上问题的解决，进一步阐明反复自然流产的发病机制，为临床反复自然流产的治疗提供新的思路。

Accumlating evidence already shows that microRNAs are small non-coding RNA which are involved in cell differentiation,development and the occurrence and pathogenesis of many diseases. At present study, first, we did the micro-array for microRNA expression in deciduas and villi from recurrent spontanous abort patients.it showed that the expression profiles of microRNAs were significantly difference between that in deciduas or villi in normal abortion patients and that in deciduas or villi in recurrent spontanous abortion patients.Espacially, the expression of mir-184 both in deciduases and villi in recurrent spontanous abortion patients were significantly higher than that in deciduases and villi in normal abortion patients. Prediction of the target genes of mir-184 suggest that mir-184 was closely associated with the cell development, differentiation and immune response.In order to extensively to explore the function of mir-184 in recurrent spontanous abortion, further experiments including mir-184 function on proliferation, invision, apoptosis and protein secreting profile of trophoblast are going to be conducted.