胶质瘤具有沿着白质纤维等特定解剖结构侵袭的特点。近期发现，神经元释放的神经营养因子可使胶质瘤细胞获得生长优势。但并非所有的胶质瘤细胞靠近神经纤维后即停止迁移并开始增殖，部分胶质瘤细胞会沿着白质纤维侵袭到远处并形成新的肿瘤灶，其具体机制不明。根据文献、数据库分析及课题组前期预实验结果，我们发现前神经型胶质瘤干细胞（PN-GSC）具有更强的沿白质纤维侵袭的能力且高表达间隙连接蛋白43。因此我们推测：PN-GSC亚群可与神经元形成异源型间隙连接，促进其沿着白质纤维侵袭。本研究拟用神经元与PN-GSC体外共培养模型、动物模型和胶质瘤病人标本观察体内外异源型间隙连接的形成情况，及其在调控PN-GSC沿着白质纤维侵袭中的作用，并评估临床诊断治疗意义。研究结果有助于深入认识胶质瘤侵袭性生长的发生机制，进而为探寻胶质瘤的诊疗靶点提供新的实验依据。

Invading along specific anatomical structures such as white matter fiber is the most remarkable invasive characters of glioma. Recent studies have found that neurotrophic factors released by neurons can provide growth advantages for glioma cells. And crucially, unlike most cancer cells, when nearing to nerve fiber, some glioma cells can invade along white matter fiber and metastasis to distant areas instead of settling down. But the specific mechanism is unclear. According to the literature, database analysis and preliminary results of our research team, we found that proneural glioma stem cells have stronger ability to invade along white matter fiber and express higher gap junction protein 43. Therefore, we speculate that: heterologous gap junction channels formed between the neuron and proneural glioma stem cell, and invade along the white matter fiber. This study will investigate the formation of heterogeneous gap junction channels between neurons and proneural glioma stem cells, its role in regulating glioma cells invasion along the white matter fiber, and evaluate the significance of clinical diagnosis and treatment by co-culture neurons with proneural glioma stem cells, animal models and glioma patient specimens. The obtained results of the study will not only helpful for understanding the mechanism of the aggressive growth of glioma, but also provide a new experimental basis for exploring the diagnostic and therapeutic targets of glioma.