既往研究发现端粒长度和衰老密切相关，而POT1是端粒核心功能蛋白“Shelterin”复合体的重要组成部分，其单核苷酸多态性位点和衰老相关。我们推测POT1的另一种基因多态性—拷贝数变异（copy number variation，CNV）也可能参与衰老的进程。因此，本研究通过检测人群中POT1基因的CNV情况，评价POT1基因的CNV与衰老的关系；构建含有POT1基因的质粒并转染入WI-38细胞，同时RNAi POT1基因，观察POT1的mRNA和蛋白表达情况，通过检测细胞周期和衰老相关表型染色，评价POT1基因对WI-38细胞衰老的影响；将筛选出来的目标CNV和其他标志物一起，使用Klemera and Doubal法构建生物学年龄积分公式，为更加准确的评价个体衰老程度提供科学依据。

Previous studies have found that telomere length and is closely related to aging, and POT1 is an important part of the core functions of telomere protein "Shelterin" complex, which single nucleotide polymorphisms associated with aging. We speculate that another gene polymorphism POT1 - copy number variation (CNV) may also be involved in the aging process. Therefore, this study detected CNV of POT1 gene evaluation POT1 gene CNV and aging; construction of plasmid containing POT1 gene and transfected into WI-38 cells, and RNAi gene POT1, POT1 observed mRNA and protein expression of case, the cell cycle and senescence-related phenotypes staining impact assessment POT1 gene on WI-38 cells senescence; will filter out the target CNV and other markers take together, use Klemera and Doubal method constructed biological age integral formula for a more accurate evaluation of the degree of aging individuals to provide a scientific basis.