野猪对森林地被层翻拱干扰逐年加重，土壤结构、理化性质和水文条件均受到严重影响，而对土壤生物群落组成和功能的影响却鲜见报道。森林土壤生物群落组成复杂，对诸如野猪翻拱的干扰极为敏感，研究单一类群难以澄清其对该干扰的响应机制。本项目以温带典型植被阔叶红松林为研究对象，采用网络分析理念和技术，将土壤生物群落作为有机整体考量，通过对翻拱迹地和未翻拱样地比较，研究野猪翻拱对土壤微生物和主要动物群落组成和活性的影响，探明土壤生物各分类单元间的共存关系；结合15N稳定同位素示踪、探针技术和巢式标签DNA宏条形码技术，剥离出营养型互作关系，构建土壤N同化网络。提出野猪翻拱干扰情景下的森林生态系统土壤氮同化网络模式，阐述翻拱对土壤氮同化网络拓扑结构和功能的作用机制，探讨土壤氮同化网络结构与氮同化功能的关系，为评估大型动物对森林生态系统氮循环影响提供科学依据。

Wild boar grubbing has been reported to affect soil physical, hydrological and chemical properties seriously, and its influence on the composition and function of the soil biome is still little known. The composition of forest soil biological community is extremely complicated, and it is very sensitive to disturbance. It is difficult to clarify its response mechanism to wild boar disturbance. This project will select the temperate broad-leaved Korean pine forest that is a typical vegetation as the research object and measure soil biological community composition periodically in the boar disturbed and undisturbed plots. Combining with the determinant of soil physical and chemical properties, biological community activity and nitrogen assimilation process using network analysis theory and technology, we will plan to know the relationship between soil biological interactions between each taxon clearly. After determining the trophic relationship between all kinds of group, we will build the soil N assimilation network and give a model that represents soil nitrogen assimilation network of the forest ecosystem by wild boar grubbing. We will explore the mechanism of grubbing disturbance on soil nitrogen assimilation network topology structure and function, and document the relations of the network structure and the function of nitrogen assimilation. Our results would provide scientific evidence for evaluating the nitrogen cycle and large-scale animal management of forest ecosystem in China.