脱氧雪腐镰刀菌烯醇（deoxynivalenol, DON）在食品中的污染比例及检出率较高，是食品安全管理中日常检测、监测及控制的重点。但由于各种复杂生物基质的干扰，导致各种检测方法均存在提取效率低、检测灵敏度低、回收率低和稳定性差等缺陷。因而研究高选择性、高特异性的新型吸附材料及样品前处理方法至关重要。本项目创新性地以Fe3O4@Au纳米颗粒为载体，仅需少量模板分子，通过磁流体、表面分子印迹等方法，制备尺寸小、比表面积大的磁性分子印迹聚合物（M-MNPs）纳米颗粒。通过物理、化学等多种分析手段定量、系统研究M-MNPs的结构、比表面积、热力学、结合特性、选择特性等基础特性，优化印迹体系；同时利用核磁共振、红外光谱、紫外光谱等多种手段揭示DON M-MNPs与靶标DON分子的识别机理，明确起识别作用的重要功能基团。本项目研究结果将为新型分子印迹聚合物在真菌毒素实际检测中的应用提供理论依据。

Mycotoxin deoxynivalenol (DON) is seriously contaminated and frequently detected in foods and agricultural products, and thus is always paid significant attention on its routine analysis, monitoring and control within the whole framework of food safety administration.Practically,the existence of complicated biomatrices in sample preparation leads to the unavoidable drawbacks of low-efficiency during extraction procedure, as well as low detection limit, recovery and stability with regard to related applied analytical methods. Therefore, development of highly specific and selective adsorbents as fundamental materials in sample preparation is extremely important to overcome the above mentioned unsuffciencies, which is also under indeed needs there. In this proposal, using the smallest quantity of expensive DON as template molecule, novel magnetic molecularly imprinted polymer nanoparticles (M-MNPs) via surface imprinting against the target mycotoxin is firstly prepared. As designed,M-MNPs are expected to have the advantages of less volume and larger surface area, with Fe3O4@Au nanoparticles as supporting. Then, the nano-structure, specific surface area, selectivity, adsorption kinetics and thermodynamics characteristics of the obtained M-MNPs are thoroughly studied by multiple analyses via physical and chemical approaches, with optimization on the imprinting components. Furthermore, the recognition mechanism between the target molecule of DON and the M-MNPs are elucidated by the means of Nuclear Magnetic Resonance (NMR),Fourier Transform Infrared Spectroscopy (FTIR) and UV-visible spectroscopy, to reveal the key functional chemical groups responsible for the recognition steps. As resulted, the achieved data of this current research work will provide new funcational materials of specific adsorbents for preconcentration and clean-up of sample preparation.More importantly, the discovered theoretical evidences of recognition mechanism between the target DON and M-MNPs will open new avenues to substantially support the wide application of molecularly imprinted polymers as specific adsorbents for mycotoxin analyses in practical uses.