农业面源污染一直是困扰我国的重要环境问题。木屑生物反应器（WBR）能高效去除农业废水中硝酸盐，但生物淤积和反硝化性能衰减仍限制其应用。AHLs类群体感应淬灭（QQ）可有效控制生物反应器中生物淤积，且生物炭（BC）具有提高WBR反硝化性能的潜力。本项目拟采用BC固定QQ菌（BC/QQ），应用于WBR以同时调控其生物淤积和反硝化性能。然而，尚无该体系应用于WBR的报道，相关作用和机理亟需探究。本项目对比分析不同WBR的水力性能、滤料特性、硝酸盐去除及释碳性能，构建相关模型，并检测反硝化功能基因，阐明BC和QQ对WBR反硝化性能的协同作用和增强机制；研究QQ酶活性、微生物群落特征、生物膜、污泥特性和AHLs浓度的变化规律，从微生物和分子水平上探究BC/QQ抑制WBR生物淤积的可行性和微观机理。研究结果有望为BC协同QQ调控WBR性能提供理论和实践依据，为确保农业废水中硝酸盐的长效去除提供新途径。

Agricultural non-point source pollution has always been an important environmental problem in China. Although woodchip bioreactors (WBR) can efficiently remove nitrate from agricultural wastewater, biofouling and denitrification efficiency degradation still limit its further application. AHLs based quorum quenching (QQ) can effectively control the biofouling in the bioreactor. At the same time, biochar (BC) has the potential to improve the denitrification performance of WBR. Therefore, this project intends to immobilize QQ bacteria using BC (BC/QQ), and apply it to WBR to regulate its biofouling and denitrification performance simultaneously. However, there is no report about the application of this system to WBR, and the related function and mechanism need to be explored urgently. This project aims to compare and analyze the hydraulic performance, filtering media characteristics, nitrate removal and carbon release performance of different WBRs, and to build related models, as well as to detect denitrification functional genes, so as to clarify the synergistic effect and enhancement mechanism of BC and QQ on denitrification performance of WBR. In addition, the feasibility and microscopic mechanism of biofouling control in WBR using BC/QQ were explored on the microbial and molecular levels by studying the changes of QQ enzymic activity, microbial community characteristics, biofilm characteristics, sludge characteristics and AHLs concentration in WBR. The results are expected to provide theoretical and practical basis for application of BC combined with QQ to regulate WBR performance, and provide a new way to ensure the long-term removal of nitrate in agricultural wastewater.