基于MicroPET棕色脂肪显像技术的2型糖尿病的调控因素及疗效评估的研究

针对2型糖尿病的高发和难治性，本课题将利用糖尿病小鼠模型，通过小动物正电子发射断层显像（microPET）研究，从激活棕色脂肪(BAT)的角度探索解决途径。研究内容包括：①动态观察不同药物调控下糖尿病小鼠BAT对于18F-FDG摄取的变化，分析影响BAT 功能的因素及与糖尿病鼠生化指标的内在联系，建立准确、定量评估BAT 功能状态的可靠方法；②动态观察BAT移植术后糖尿病鼠的BAT活性及疾病变化，探讨BAT移植在疾病发展过程可能的作用。③通过系列MicroPET 68Ga-NOTA-Exendin-4显像，评估糖尿病鼠胰岛功能的变化，并建立与BAT功能的相关性。本课题将利用microPET 这一分子影像工具，揭示BAT 功能状态与糖尿病病程的内在联系，为糖尿病相关研究提供重要的机体功能信息及实验证据，探索治疗糖尿病及疗效评估的新思路，并建立相应的基于先进影像技术的研究手段和评估方法。

The high incidence and treatment strategy for type 2 diabetes mellitus(DM) has been challenging for doctors and scientists all over the world. The aim of this project is to explore a novel way for type 2 diabetes mellitus treatment by activating brown adipose tissue (BAT) in mouse models with small animals positron emission tomography (microPET). The main research contents include three parts: 1. Observe dynamically how different factors regulate the 18F-FDG uptake of BAT in diabetic animal models, study the relevance of BAT to biochemical parameters，and set up an accurate and quantitative modality for evaluating the function status of BAT. 2.Analyze the dynamic change of BAT function before and after BAT transplant surgery, and explore the role of BAT transplantation in the development of DM.3.Evaluate the pancreatic function with MicroPET 68Ga-NOTA-Exendin-4 imaging, and investigate the relativity between BAT and pancreatic function status. In this project, we will try to reveal the inner link between BAT and DM, provide important information of body function and experimental evidance, as well as promote the further study on new modalities for treatment strategy for DM. Based on this research, we hope to contribute to establishing a comprehensive diagnosis and treatment pattern for DM with microPET and PET/CT, the advanced molecular imaging technology.