肺炎支原体(MP)具有广泛致病性，重症MP肺炎（SMPP）逐年升高。SMPP与MP耐药、毒素释放、黏液多、高凝等有关，肺上皮-间质转化（EMT）是其发病新机制。在前期燥伤肺津基础上，从燥毒论治SMPP，提出“燥毒劫伤肺络”与SMPP肺EMT调控通路相关假说。经典名方养阴清肺汤临床疗效确切，甘润苦泄，养阴清肺，兼散疫毒；热甚者，加银花、连翘；采用HPLC-MS技术，从活性成分定量、血清药理学-药物化学关联分析，探索药效物质基础。前期对MP感染小鼠肺mRNA测序筛选，发现TGF-β、IL-17 、TLR等信号通路富集显著,恰与EMT相关。高载量MP感染BALB/c小鼠建立SMPP模型，采用iTRAQ、ELISA、qPCR等方法，观察TGF-β1/Smad3信号通路诱导EMT动态变化，阐明“燥毒”、“肺络”生物指标内涵，探讨养阴清肺汤加味效果差异性，揭示其改善SMPP肺EMT的调节机制。

Mycoplasma pneumoniae (MP) has wide pathogenicity. Severe mycoplasma pneumoniae pneumonia (SMPP) cases increase year by year. SMPP is highly related to MP characteristics for its antibiotic resistance, toxin release, mucus hypersecretion and hypercoagulability. Pulmonary epithelial-mesenchymal transition (EMT) is a new found pathogenesis of SMPP. On the basis of previous studies on dryness-heat injuring lung-fluid, the hypothesis that diagnosis and treatment for SMPP by dryness-toxin is drawn. And dryness-toxin rob lung collaterals is related to epithelial-mesenchymal transition in SMPP. Yangyin Qingfei Decoction is the traditional famous formula and has exact effect in clinics, pleasant embellish bitter drain, has function that can nourish yin to clear away the lung-heat and eliminate epidemic toxin, while adding honeysuckle and forsythia has benefit to patients with high fever. We applied the techniques of HPLC-MS to combine active ingredients quantification, serum pharmacology as well as serum pharmacochemistry in correlation analysis to explore material basics. Previous studies found that TGF-β, IL-17, TLR and other signal pathways were significantly enriched and related to EMT. Balb/c mice infected by high MP load is designed to observe dynamic change of EMT induced by TGF-β1/Smad3 signaling pathway with techniques of iTRAQ, ELISA, qPCR and so on. This study attempted to explore the content of dryness-toxin and lung collaterals through biological indicators, to compare effectiveness of modified Yangyin Qingfei Decoction, as well as to discuss the mechanism of integrated regulation through pulmonary EMT in SMPP.