膝痹（膝骨性关节炎）是中老年高发难治性疾病，严重影响患者的日常生活和工作。本课题之陈元膏源自《千金翼方》苍梧道士陈元膏的记载，经临床化裁加减、添加维药苏仁江，使用现代工艺制备而成。“膏摩法”是将外用中药膏与推拿手法相结合的中医外治疗法，前期临床发现陈元膏摩法治疗阳虚寒凝型膝痹效果显著。现代研究表明膝痹的发生发展是涉及多条信号通路的复杂调控，但是其作用机制尚不清楚。本研究拟以新西兰大白兔为研究对象，以PI3K/AKT信号通路为调控目标，立足新疆地区阳虚寒凝证型高发的特点，以石膏固定+激素+冷泳法制作兔阳虚寒凝型膝痹模型，经陈元膏摩法干预后，以免疫组化法、HE及WB法为研究方法，观察陈元膏摩法影响膝痹发展的关键因子，探索陈元膏摩法经PI3K/AKT信号通路调控兔阳虚寒凝型膝痹软骨降解的作用机制，进而为临床使用陈元膏摩法治疗膝痹病提供实验依据。

Knee paralysis is high-risk refractory in the elder,which impact on daily life and work seriously,western medicine for knee osteoarthritis. This topic of chenyuan ointment rubbing use the pale of the "qian jin yi fang”,via the experience of clinical add or subtract, add Su Renjiang cutting,preparation and use of modern technology.So far, At present the study found that the knee paralysis development involving multiple complex regulation of signaling pathwanys. sprevious research found that chenyuan ointment rubbing of treatments for yang deficiency type haemorrheological nature knee paralysis is remarkable,but if via PI3K/AKT pathway regulateng the yang haemorrheological nature and mechanism of action is still unclear.New Zealand rabbits as the research object in this study,based on characteristics of high incidence syndrome in Xinjiang area,this topic proposed a plaster cast method and cold water immersion method for the yang haemorrheological nature,via chenyuan ointment rubbing intervention,using Ellisa,HE and WB monitoring method,approach chenyuan ointment rubbing from PI3K/AKT signaling pathways regulating the yang haemorrheological nature knee cartilage degradation mechani-sm of action,and then provide basis experimental for chenyuan ointment rubbing treat knee paralysis.