bFGF对冷冻卵巢组织体内外再生和卵泡发育的作用及机制研究

卵巢组织体内、体外再生技术限制了卵巢组织冷冻保存技术在临床生育力保存中的应用。其核心问题是体外培养或移植过程存在原始卵泡活化障碍，导致卵泡发育能力下降。bFGF的经典作用是促进血管再生，亦有诱导原始卵泡活化功能。本研究一方面利用小鼠卵巢组织体外培养方法探讨冷冻保存本身是否影响bFGF的表达、外源性bFGF对冻存卵巢组织中原始卵泡活化的作用及可能的信号通路。另一方面通过小鼠卵巢组织冷冻移植实验，探讨移植后bFGF单独作用或联用KL后对移植后血管化和卵泡发育的影响，最终通过冷冻卵巢组织移植-取卵行IVM-体外受精胚胎移植实验，观察冷冻卵巢组织移植移植后获卵、受精能力和胚胎发育及子代出生率，探讨bFGF对冷冻卵巢组织体内外再生和卵泡发育的影响。本研究将推动卵巢组织冷冻保存生育力到恢复生育力的技术衔接和理论发展。

Inefficient ovarian tissue regeneration in vitro or in vivo inhibited the clinical practice of ovarian tissue banking in female fertility preservation. The core question is the poor folliculogenesis resulted from inactivation of primordial follicles during in vitro culturing or tissue transplantation. It is known that bFGF plays an important role both in angiogenesis and also in primordial follicle activation in mammals. In the first part of this study, frozen-thawed mouse ovarian tissue will be cultured in vitro. bFGF's expression and its effect in primordial follicle activation as well as its possible molecular mechanism will be investigated. In the second part, during frozen-thawed mouse ovarian tissue allogeneic transplantation, the effect of bFGF alone or combined with KL on angiogenesis, primordial follicle activation and follicle development will be invested. IVM-IVF-ET and live birth are also involved to access the role of bFGF on fertility restoration. This study will promote the technical cohesion of fertility realization to ovarian banking.