天然源抗植物病毒活性物质是高效植物病毒病防治药剂的重要来源，受天然产物分子固有结构及理化性质的限制，其结构多样性衍生往往难以实现。为了突破天然产物固有骨架的限制，使分子结构更加简单和多样化，本项目借鉴前期β-咔啉类抗植物病毒高活性衍生物的创制研发经验，以色氨酸生物活性研究的初步结果为基础，系统开展以生源合成前体色氨酸为基本原料的结构多样性导向的绿色高效植物病毒病防治新药剂的创制研究。选取多种吲哚生物碱生源合成重要前体色氨酸作为基本原料，设计合成具有结构多样性的并环及螺环吲哚类天然产物，发展高效构筑并环及螺环吲哚分子骨架的合成方法，创建吲哚类天然产物库，对其抗植物病毒活性和构效关系进行研究，对优选的化合物进行抗植物病毒作用机制探索和毒性试验及田间小区试验。该项目对创制具有我国自主知识产权的易于合成的有开发价值的新植物病毒防治剂具有重要的理论和实际意义。

Natural source anti-plant virus compounds are one of the important sources of antiphytoviral agents. Due to the limitation of the intrinsic structure and the physicochemical property of natural product, their derivatization is often difficult to achieve. In order to break through the limitation and make the molecular structure more simple and diverse, and drawing on the experience of β-carboline antiviral inhibitor and the preliminary results of tryptophan biological activity, this project intends to systematic study the diversity-oriented green and efficient new antiphytoviral agents discovery research using biosynthesis precursor tryptophan as basic material. In this project an important biosynthetic precursor of indoles alkaloids, tryptophan, will be selected as the synthetic precursor in the design and synthesis of pseudo fused and spiro-indole natural products with the structural diversity, efficient synthetic methods of building the fused and spiro-indole molecular skeleton will be developed, pseudo indole natural products library will be created and their anti-plant viruses activities and structure-activity relationship will be studied, the antiviral mechanism of the lead compounds will be studied and their toxicity and field plot activity will be tested to evaluate their application. This project has important theoretical and practical significance for the development of new antiphytoviral agents with independent intellectual property rights and exploration value.