铝（Al）毒害是酸性土壤上限制作物生产的主要因子之一。探讨植物激素如何调控植物铝毒害和耐性机理的研究，具有非常重要的科学理论意义。我们前期研究发现BR信号参与了对铝诱导根系ALMT1表达和铝毒害的调控。本项目拟在前期研究的基础上，结合生理、生化、细胞学、遗传学及分子生物学等实验技术和手段，继续深入探讨铝胁迫下BR信号调控ALMT1表达的分子机理，阐明铝胁迫下BR信号通路如何通过作用于ALMT1的上游调控因子，以及如何与DELLA介导的GA信号通路互作来调控ALMT1的表达。筛选BR信号和DELLA介导的GA信号通路下游共同调控的关键转录因子或调节蛋白，明确其在铝胁迫下ALMT1表达调控中的作用，为进一步明确植物铝毒害和耐性调控机制，最终培育适应酸性土壤生长的作物新品种提供科学理论依据。

Aluminum (Al) toxicity is one of the major limiting factors for crop production in acid soil. It is scientifically important to study the regulatory mechanism of phytohormones in aluminum toxicity and resistance of plants. We previously have found that brassinosteroid (BR) signaling is involved in the regulation of ALMT1, a gene encoding malate transporter, and Al-induced inhibition of root growth. To further better understand the molecular regulatory mechanism of BR signaling in Al-induced expression of ALMT1, the physiological, biochemical, cellular, genetic and molecular approaches will be combined in the present study, and will clarify how BR regulates ALMT1 expression through the regulation of WRKY46, STOP1, CAMTA2 and DELLA, and also will obtain the key transcription factors or regultory proteins and will clarify their molecular and physiological functions in the regulation of Al-induced ALMT1 expression.