膜转运相关蛋白Patellin1参与调节植物耐盐性的分子机制研究

土壤盐碱化对粮食生产与生态安全构成极大威胁，理解植物耐盐机制可为遗传改良作物、提高盐碱地的利用效率等提供重要的科学依据。已知质膜Na+/H+反向转运蛋白SOS1参与调节盐胁迫下植物体内的离子平衡，但其定位与活性是如何被膜转运过程所调节的则尚不清楚。本项目前期工作发现膜转运相关蛋白Patellin1（PATL1）可与SOS1互作并参与调节植物的耐盐性。PATL1可能影响植物细胞内的膜转运过程进而调节SOS1的定位与活性，从而调节盐胁迫下植物体内的离子平衡。本项目拟通过多种手段系统分析PATL1对膜转运过程及SOS1功能的影响，阐明其调节植物耐盐性的分子机制，进一步认识膜转运过程、SOS途径及植物耐盐性三者之间的关系。

Soil salinity is one of the most serious factors limiting the productivity of crops. Understanding the mechanisms of plant salt tolerance will definitely promote the generation of gentically-engineered salt-tolerant crops. SOS1, an major component of Salt-Overly-Sensitive (SOS) pathway, is a plsama membrane-localized Na+/H+ antiporter functioning in Na+ exclusion during salt stress. However, regulation of SOS1 function by membrane trafficking is no clear. Our previous work demonstrated that a membrane trafficking-related protein, Patellin1 (PATL1) interacts with SOS1, and patl1 mutant displays salt-sensitive phenotype. Data showed that PATL1 is involved in regulating membrane trafficking. In addition, cellular ion homeostasis might be disrupted in palt1 mutant. We will elucidate the mechanisms by which PATL1 modulates membrane trafficking and SOS1 distribution and activity in response to salt stress. Our goal is to determine the connection between membrane tranfficking and salt tolerance in plants.