油脂是广泛应用于动物生产中的一种高能饲料原料，但其在储存、加工和饲喂过程中易发生氧化酸败，从而使动物发生氧化应激，进而危害动物健康和生产性能。目前尚缺乏关于氧化油脂对母猪乳腺损伤及子代氧化应激的影响和分子机制以及有效干预手段研究。本项目拟首先研究不同程度氧化豆油和各级氧化产物对体外培养乳腺上皮细胞凋亡及相关代谢通路基因表达的影响，阐明危害的主要代谢产物及浓度；其次利用建立的乳腺上皮细胞损伤模型筛选生物抗氧化剂，研究其对氧化油脂致母猪乳腺细胞损伤的缓解作用；最后选取妊娠后期母猪开展动物试验，通过检测母猪胎盘、乳中油脂氧化产物的残留量和仔猪组织氧化应激相关分子信号通路的变化，阐明氧化油脂对母猪乳腺及子代氧化应激的影响和作用分子途径，同时验证生物抗氧化剂是否具有缓解功效。试验结果对深入了解氧化油脂对母猪和子代的危害、分子机制，建立有效的干预措施提供了新思路和实验依据。

Oil is a high-energy feed which is widely used in animal production, however, in the process of storage, processing and feeding, it is prone to oxidative to cause animal oxidative stress and endanger animal health and performance. At present, there is still a lack of research on the effects, molecular mechanism and effective intervention methods of sows mammary gland damage and progeny oxidative stress caused by oxidized oil. Firstly, this project intends to study the effect of different-content oxidized soybean oil and its peroxidation products on the apoptosis of porcine mammary epithelial cells in vitro and gene expression of related metabolic pathway, clarify the harm of main metabolites and its concentrations. Secondly, this study intend to investigate the effect of bioantioxidants which ease the damage of mammary gland cells caused by oxidized oil according to the established damage cell model. Finally, we plan to illustrate the effect of oxidative stress on sow mammary gland and their offspring, meanwhile, verify the availability of bioantioxidants by detecting the residues of peroxidation products in sows placenta and milk and the change of offspring oxidative stress-related signaling pathways through conducting animal experiment during the late of gestation to lactation of sows. We predict that the results of this project will provide a new idea and experimental basis for understanding the harm and molecular mechanism of the sow and offspring caused by oxidized oil, and establishing the effective intervention measures to strengthen the nutrition of sows.