基于多肽自组装的耦合增强SERS生物传感新方法及活细胞蛋白酶活性成像研究

蛋白酶具有重要的生物调控作用，在细胞体系的研究中占极重要地位。发展高灵敏、高通量的细胞内蛋白酶原位检测技术是活细胞分析领域的一项挑战性任务。本项目拟将生物传感技术、耦合增强SERS技术及纳米探针技术相结合，在探索发展基于多肽自组装介导纳米颗粒团聚的耦合增强SERS生物传感新方法的基础上，构建细胞内蛋白酶活性的高灵敏、高选择性生物传感新方法，发展高分辨的原位活细胞蛋白酶活性拉曼成像分析新技术。并更进一步构建多重的SERS纳米探针，发展细胞内多种蛋白酶的高通量检测分析方法及高分辨原位成像技术，提高细胞内蛋白酶活性分析水平，以期为相关的信号传导通路研究、药物发现、疾病诊断提供有效的技术手段和平台。

Protease plays an important role in biological regulation and always attract a lot of interests in the field of cell research. It has been a great challenge to develop sensitive and high-throughput in situ cell detection methods for intracellular protease activity assay. Via combining the advangtanges of biosensor technology, coupling-enhanced SERS and nanoprobe technology, the project focuses on the development of novel coupling enhanced SERS-based biosensors on the basis of peptides self-assembly mediated aggregation of nanoparticles. Based on this technique, a coupling enhanced SERS-based assay method will be developed for high-sensitivity biosensing and high-resolution in situ Raman imaging of protease activity in living cells. Furthermore, such a strategy will be further applied for multiplexed detection and high-resolution imaging of protease activities in living cells. We believe that this strategy hold the potential as an efficient platform and applicable strategy for studies of cell signaling pathways, drug development and cell diagnostics.