甘蓝型油菜 kelch motif ACBP4 基因克隆及其功能分析

提高含油量和改善脂肪酸组分是当前油菜育种的主要目标之一。酰基辅酶A结合蛋白（ACBPs）结合酰基辅酶A酯并维持细胞内酰基辅酶A库，它们在脂质代谢，植物发育和应激反应中都有重要意义。在模式植物拟南芥中鉴定了在大小和结构域上不同的四类ACBPs，而在甘蓝型油菜中，对ACBPs的研究主要集中在ACBP6；因此，在甘蓝型油菜中研究BnACBPs功能非常有意义。本项目拟首先从甘蓝型油菜中克隆酶BnACBP4，通过在油菜中超表达和敲除来研究该基因对油菜种子含油量和脂肪酸组分的影响；对获得的转基因株系进行转录组分析，通过研究差异表达基因参与的代谢途径而揭示ACBP4引起含油量和脂肪酸变化的原因。

Increasing the oil content and improving the fatty acid compositions are one of the main breeding project in Brassica napus. The acyl-coA binding proteins (ACBPs) bind acyl-coA esters and preserve the intracellular acyl-coA pool. Their significant implication in lipid metabolism, in plant development and stress response highlight their importance. In B. napus, researches on ACBPs were mainly focused on ACBP6. It would be of a great importance to analyze the function of these BnACBPs in B. napus. Thus, the aim of this project is to clone BnACBP4 and to perform the function analysis by using of overexpression and knock out techniques, and to analyze the effects of these genes on oil content and fatty acid compositions. The differentally expressed genes would be analyzed on the transgenetic materials in order to obtain the inner reasons why ACBP4 could lead to the change of oil content and fatty acid compositions.