环境水体中多种类固醇雌激素的现场快速荧光分析

类固醇雌激素(SE)是一类以类固醇环为基本核的环境内分泌干扰物，广泛存在于环境水体中，对人类健康和生态系统构成严重威胁。目前常规方法均难以满足环境水体中多种SE现场快速灵敏检测的需求。本项目拟以环境水体中危害最大的四种SE为研究对象，利用多个不同核酸适配体、抗体或分子印迹聚合物识别材料对多种SE的交叉反应性质，系统开展多种SE动态组合库高灵敏荧光传感新方法的研究，并探索该方法在环境水体现场快速分析中的有效性。研究内容包括：1)不同荧光标记识别材料的获取或制备及表征；2)识别材料与SE之间的相互作用研究；3)不同识别材料的动态组合库荧光传感策略的建立及传感策略中其它材料的设计和功能化；4)结合化学计量学建立多种SE检测的荧光分析新方法；5)新方法在环境水体现场快速分析中的有效性评价。本项目的实施不仅推进现场快速荧光传感新方法的发展，而且还将促进传感分析、环境分析和化学计量学的深度交叉和融合。

Steroid estrogens (SE) are a kind of environmental endocrine disruptors with steroid ring structure. They are widely found in environmental water, and pose serious threats on human health and ecological system. At present, it is very difficult by using routine approach to meet the requirements for fast, on-site and sensitive detection of multiple steroid estrogens. In the project, we will select the four most harmful steroid estrogens in environmental water as analytes, and exploit the cross-reactive property between several different nucleic acid aptamers, antibodies or molecularly imprinted polymers and different steroid estrogens to systematically carry out the investigations of the new fluorescence method based on dynamic combinatorial library for the detection of multiple steroid estrogens. In addition, we will explore the effectiveness of the new method for fast on-site detection of multiple steroid estrogens in environmental water. The study includes five parts: 1) the collection, preparation and characterization of different fluorescently labeled recognition materials; 2) the investigations on the interactions between recognition materials and steroid estrogens; 3) the establishment of florescence sensing strategy based on dynamic combinatorial library and different recognition materials, and the design and functionalization of other materials in the sensing strategy; 4) the establishment of new fluorescence analysis method coupled with chemometrics for the detection of multiple steroid estrogens; 5) the evaluation on the effectiveness of the new method for fast on-site detection of multiple steroid estrogens in environmental water. The implementation of the project will advance the development of new methods for fast on-site fluorescence sensing, and promote the deep crossing and integration in the field of sensing analysis, environmental analysis, and chemometrics.