炎症反应是脑缺血再灌注损伤（CIRI）重要因素之一，但炎症激活的机制尚未完全阐明。小胶质细胞（MG）是免疫系统的一部分，在神经系统中具有多种功能，在CIRI与修复中具有重要作用，目前已经成为神经系统疾病的研究热点。我们前期工作中发现，CIRI过程中MG能够表达树突状细胞（DC）特异性标志，说明MG能够转化成DC（称之为DC样细胞）；同时，证实脑损伤面积与MG转化成DC样细胞的数量呈正相关，提示MG转化成DC样细胞可能参与脑损伤的病理过程，该发现国内外尚未见报道。是什么因素导致MG转化成DC样细胞的？由MG转化而来的DC样细胞是否具有DC的功能？本课题通过体内外实验，阐明CIRI过程中MG转化成DC样细胞的机制；明确DC样细胞对神经细胞损伤、血脑屏障开放及炎症细胞浸润的影响及其机制。阐明这些问题，将丰富脑缺血再灌注免疫二次损伤机制，为临床防治CIRI提供新的策略和新的靶点。

The inflammatory reaction is one of the key factors in cerebral ischemia-reperfusion injury (CIRI), however, the mechanisms of this inflammatory activation have not been fully elucidated. Microglia (MG) is a member of immune systerm, having multiple functions in nervous system, and playing an important role in CIRI and its repair, so far it has been becoming a central issue. In our preliminary work, we found that MG could express the dendritic cells (DC) marker during the process of CIRI, indicating MG has the potential of converting to DC (known as DC-like cells). Meanwhile, we confirmed the injury area of the brain was positively related to the cell numbers of DC converting cells from MG, suggesting the converting process from MG to DC-like cells might participate in the pathological change of brain injury, which has not been reported yet. While, what are the mechanisms, and whether these DC-like cells perform DC functions? The purpose of this research is to clarify the mechanisms of the generation of DC-like cells in CIRI, and the roles of these DC-like cells in nerve cell injury, blood brain barrier opening and infiltration of inflammatory cells via both in vivo and in vitro tests. Our research will enrich the mechanisms of the secondary immune cerebral ischemia reperfusion injury, providing a new strategy and targets for the clinical prevention and treatment of CIRI.