动脉粥样硬化（Atherosclerosis，AS）发生发展的机制是心血管研究领域最重要的课题之一。近期研究发现Erbin作为细胞骨架蛋白，参与调控血管新生，而血管新生是影响AS发生发展的重要机制。我们前期研究观察到Erbin在人冠状动脉斑块中主要表达于内皮细胞，并促进内皮细胞迁移和成管，提示Erbin可能通过调控血管新生参与AS的发生发展。课题组收集人冠脉标本观察Erbin表达水平与斑块内新生血管及AS的相关性；通过构建Erbin干扰及高表达慢病毒转染ApoE-/-小鼠，并培育Erbin-/-ApoE-/-双敲小鼠，明确其在整体动物水平对血管新生及AS影响；继而通过体外实验明确Erbin对内皮细胞增殖、凋亡、迁移、成管等影响；最后通过检测多条信号通路明确其发挥生物学作用的分子生物学机制。所得结果可以揭示Erbin对AS发生发展的影响，更可为AS的防治提供新的干预靶点和实验依据。

Investigating the mechanisms of the development of atherosclerosis has become one of the most urgent topics in the field of cardiovascular research. Recent studies have shown that as a cytoskeleton protein, Erbin has been involved in the regulation of neovascularization, which has been seen as one of the most important mechanism regulating the development of AS. Recently, we observed that Erbin was mainly expressed in endothelial cells in human coronary artery plaque, and promoted the migration and tube formation of endothelial cells, suggesting that Erbin may be involved in the development of AS by regulating neovascularization. The research group intends to firstly observe the correlation between Erbin expression level and intra-plaque neovascularization and AS through the collected human coronary artery; through construction of Erbin interference and over-expression lentiviral and transfection to ApoE-/- mice, and cultivation of Erbin-/-ApoE-/- double knockout mice, we can clarify the influence of Erbin on neovascularization and AS in animal level; then through in vitro studies, we can illuminate the effects of Erbin on endothelial cell proliferation, apoptosis, migration and tube formation; finally through detection of multiple signaling pathways we can illustrate the specific molecular mechanism. The results can not only reveal the effect of Erbin on the occurrence and development of AS, but also provide a new intervention target for prevention and control of atherosclerotic disease.