心室重构是CHF发生发展的重要病理生理学基础，心室重构过程中神经内分泌细胞因子的激活与IKK/IκB/NF-κB信号通路关系密切，阻断IKK/IκB/NF-κB信号通路，是阻抑心室重构、防治心衰的重要策略之一。中医认为，CHF属本虚标实之证，心阳（气）亏虚贯穿CHF发生发展的始终，温阳益气乃其基本治法。既往研究表明，温阳益气代表方苓桂术甘汤能够抑制CHF模型大鼠神经内分泌炎性细胞因子BNP、AngⅡ、AT1R、Ald、ET-1、TNF-α、IL-6、IL-1β和核转录因子NF-κB过度激活、阻抑心室重构、改善心功能。本项目拟在前期基础上，采用体内外相结合的研究方法，运用Western blot、RT-qPCR、ELISA、免疫荧光、激光共聚焦技术和流式细胞术等现代分子生物学技术，从IKK/IκB/NF-κB信号通路入手，深入探讨苓桂术甘汤干预心室重构的分子机制，为该方临床应用提供实验依据。

Ventricular remodeling is the important pathophysiology foundation in the occurrence and development of chronic heart failure（CHF）. IKK/IκB/NF-κB signal pathway is closely related to the activation of neuroendocrine inflammatory factor of ventricular remodeling after myocardial ischemia, and it is an important target for intervention of ventricular remodeling. Traditional Chinese Medicine believes that CHF belongs to the syndrome of deficiency of Ben and repletion of Biao. Deficiency of Heart Yang and Qi runs through the occurrence and development of the whole process of CHF. Warming-yang and benefiting-qi is the basic therapeutic methods of CHF. The previous findings showed, as the representive prescription of warming-yang and benefiting-qi, Lingguizhugan Decoction could inhibit the over-expressions of the neuroendocrine inflammatory factor of TNF-α, IL-1β, IL-6, AngⅡ, Ald, ET-1, BNP and the nuclear transcription factor of NF-κB, and also inhibit ventricular remodeling of rats with CHF and improve the diastolic and systolic function of heart. Based on the preceding research, this program will use research methods of the combination of in vitro and in vivo. Serum pharmacological method, Western-blot, RT-qPCR, ELISA, immunofluorescence and flow cytometry and other modern molecular biology techniques will be used to intensively study the relationship between the effect of Lingguizhugan Decoction on ventricular remodeling and IKK/IκB/NF-κB signal pathway, find its target further reveal the biological foundation of Lingguizhugan Decoction in the prevention and treatment of CHF to provide theoretical evidence of its clinical application.