心室成形术（SVR）后的顽固性心衰及恶性心律失常仍严重威胁患者的远期预后，目前认为这与临床应用的补片密切相关。由于当前补片均为合成材料且缺乏导电能力，故在体修补后①炎性反应重，远期易僵硬、钙化；②易诱导局部形成异常折返环路。前期，研究者以导电高分子材料——聚吡咯及自体脂肪脱细胞基质为主要原料，合成了一种具有良好的导电性及组织相容性的新型修补材料。本研究将继续以该导电材料为细胞载体，在体外验证其促心肌细胞成熟及同步化收缩的能力。进而还将接种有同系大鼠乳鼠心肌细胞的上述补片应用于大鼠SVR模型中，并从形态学，组织学，功能学及电生理学等角度评判其在体生物学行为。我们期望，这种以自体来源的组织基质与纳米导电材料的结合形成的新材料能减少补片植入后的炎症反应，改善移植细胞与宿主细胞的一体化整合，进而改善修补局部组织的导电及收缩性能，从而为室壁瘤患者提供一种个体化的，具有导电性能的修补材料。

Nowadays, it remains a lethal threat for many patients underwent surgical ventricular restoration (SVR) due to the high incidence of refractory heart failure and malignant arrhythmia late after the surgery. The present patch should be partially responsible to the problem because they are synthetic and non-conductive. After implantation, the inert and non-conductive material may trigger serious inflammation and “electrical remodeling” in and along the patch, which in turn render the patch hardened and the reentant circuit formed, leading to the heart dysfunction and arrhythmia. Presently, we developed a novel conductive biomaterial by covalently conjugating polypyrrloe (a nano conductive polymer) with autologous adipose-derived extracellular matrix (A-dECM). The preliminary data demonstrate that A-dECM-Ppy patch possesses excellent biocompatibility, mechanical property and conductivity. In this project, we will continue to examine its capability to induce cell maturation and synchronic contraction by transmiting electrical signal among cardiomyocytes. Furthermore, we will seed syngeneic neonatal rat ventricular myocytes (NRVMs) onto the A-dECM or A-dECM-Ppy patch and performed SVR for immunosuppressive rats with the 2 biomaterials and their cell-free controls respectively. During the 8-week observation, we will compare the cardiac function and arrhythmic score among the 4 groups of rats. At the endpoint of the study, all the 4 groups of heart samples will be harvested and compared morphologically, histologically, or electrophysiologically. Expecially, we will focus on the integrative connection between donor and host cells and its correlation with conductive material. We hope our individualized, conductive EHT (A-dECM-Ppy+CM) would provide a better choice for those with ventricular aneurysm to deal with cardiac dysfunction and arrhythmia.