造血干细胞的增殖和分化受到转录因子的严格调控，而染色质重塑因子则是转录因子重要调控分子。但染色质重塑因子在造血干细胞增殖与分化中作用所知甚少。申请人前期工作表明在斑马鱼胚胎中敲减染色质重塑因子CHD7(chromodomain helicase DNA-binding protein 7)能促进原始和永久造血干祖细胞的增殖。另外，申请人还观察到部分斑马鱼胚胎心脏和血管中的血红细胞减少。因此，我们推测该基因可能在造血干细胞调控中扮演新的角色。本项目将利用斑马鱼为研究对象，通过基因敲除、敲减、过量表达和荧光标记等方法，探讨CHD7参与造血干细胞的增殖分化的作用及机制。本项目的展开将为进一步明晰造血机制、了解相关血液疾病致病机理和促进干细胞治疗广泛应用打下理论基础。

Current research indicates the strict regulation of proliferation and differentiation of hematopoietic stem cells (HSCs). Thus, chromatin remodeling factors are important regulator of transcription factors. However, little know about the involvement of chromatin remodeling factors in regulation of HSCs. Our preliminary experiment results indicated that knock down of CHD7 gene in zebrafish could upregulatethe the proliferation of HSCs. Furthermore, we also observed the decreasement of red blood cells in heart and blood vessels.Therefore, we speculate that CHD7 plays a new role in the proliferation and differentiation of HSCs. Our project aims to use zebrafish as the study subject，gene knock down, knock out, overexpression and fluorescence labeling are employed to investigate the molecular mechanism of the effect of CHD7 on the proliferation and differentiation of HSCs. Development of our project will be beneficial to further understanding the mechanism of hematopoiesis and pathogenesis of related disease and broad application of stem cell treatment.