聚集诱导发光笼型分子聚合物纳米粒用于活细胞内线粒体温度长期可视化的研究

线粒体作为细胞的“动力工厂”，是影响生命体内的温度变化最为关键的细胞器。对线粒体温度长期监测有助于深层次了解相关的生物过程和疾病发生。本项目利用多级荧光能量共振转移效应，构建线粒体靶向的聚集诱导发光笼型分子聚合物纳米粒，通过纳米粒的荧光颜色变化实现对线粒体温度的长期可视化监测。利用结构稳定的聚集诱导发光笼型分子聚合物纳米粒的荧光颜色变化长期监测线粒体温度，不仅避免细胞复杂环境造成荧光强度的改变导致的温度测量误差，而且可根据不同细胞对光响应差异灵活地选择所需荧光探针的发光颜色，保障对线粒体温度长期可视化监测的准确、易读和高效性。本项目的成功实施不仅可为实现活细胞内线粒体温度的可视化研究奠定基础，而且有望为疾病发生的进行早期诊断与治疗提供帮助。

Mitochondria, as the power plants of the cells, are the most important organelles that affect the temperature variation of living life. The long-term temperature visualization of mitochondria would facilitate to understand the relevant biological processes and diseases profoundly. In this project, by utilizing the förster resonance energy transfer (FRET) effect, the mitochondrial targeted polymeric nanoparticles based on aggregation induced emission cages were constructed that could achieve the long-term temperature visual monitoring of mitochondria through its fluorescent color variation. This new method will not only avoid the temperature measuring error of fluorescent intensity variation caused by the complex cellular microenvironment, but also can flexibly select the fluorescent color of nanoparticles according to photo-responsiveness of different cells, which could guarantee accuracy, legibility and efficiency for long-term temperature visualization of mitochondria. The successful implementation of this project not only lays a foundation for the visualization of mitochondrial temperature in living cells, but also may provide help for the early diagnosis and treatment of diseases.