MiR156时序协同miR164-miR319调控水稻锯齿叶矮缩病毒病症状形成机制

水稻锯齿叶矮缩病毒（Rice ragged stunt virus，RRSV）是呼肠孤病毒科（Reoviridae）水稻病毒属（Oryzavirus）的典型成员，该病毒侵染水稻诱发植物矮缩、分蘖增多和叶缘缺刻呈锯齿状等病害症状。已有研究表明RRSV能够显著诱导miR319、miR164和miR156的高表达；miR319过量表达促进病毒侵染，病害症状加重。而miR319和miR164在拟南芥中被证明与miR156-"分子时空开关"协同调控植物叶片的复杂度。本项目拟利用RRSV-水稻互作系统，采用遗传学手段初步解析miR164在RRSV诱导病害症状形成及病毒侵染过程中的生物学功能，同时探讨miR319、miR164和miR156三者如何协同作用参与RRSV胁迫下水稻叶片缺刻症状的产生过程。该项研究将初步阐释miR319-miR164-miR156在植物-病毒互作过程中的功能。

Rice ragged stunt virus, a member of the genus Oryzavirus in the family Reoviridae, infects rice and induces typical disease symptoms including stuntedness, excess tillering, and leaves with serrated edges. Previous study showed that RRSV infection highly increased the accumulation of miR319, miR164 and miR156; miR319 overexpression was responsible for the development of disease symptoms and the viral infection process. Besides, miR164 has been proved to play an important role in regulating leaf complexity through interacting with miR319 and miR156. In this project, we will apply RRSV-rice interaction system to analyze the miR164 biological function in the development of disease symptoms and viral infection process, and discuss how these three miRNAs synergistically affect leaf development upon RRSV infection. This project will indicate the role of miR164-miR319-miR156 in host-virus interaction.