MiRNA表达谱的改变可致正常成纤维细胞（NF）和肿瘤相关成纤维细胞（CAF）之间表型发生相互转变，从而进一步影响癌细胞的生物学行为；复方苦参液的抗癌作用机制可能与其抑制CAF细胞生物学功能有关。本项目拟寻找大肠癌（CRC）组织的成纤维细胞中，对其表型转化起关键作用的特异性miRNA，并验证复方苦参液治疗大肠癌的具体作用机制。本研究首先观察复方苦参液对肠癌CAF细胞活化的抑制作用，通过对比NF细胞及CAF细胞miRNA表达谱及功能学验证，找出其中影响肠癌CAF表型的关键miRNA分子；进一步通过抑制或过表达相应miRNA分子以观察复方苦参液是否通过关键miRNA分子抑制肠癌CAF细胞；最后通过对比NF细胞和CAF细胞中mRNA表达谱及荧光素酶报告基因实验探讨候选miRNA的作用途径及复方苦参液的作用机制。本研究的完成能为肠癌治疗提供新靶点，并阐明复方苦参液治疗肠癌的具体机制。

The alteration of miRNA expression profiling in fibroblasts could induce the phenotype conversion between normal fibroblasts (NFs) and cancer-associated fibroblasts (CAFs), thereby further influence the biobehavior of cancer cells. The mechanism of cancer inhibition by Combined Kushen Injection maybe due to the inhibition of CAFs biobehavior. This study will find the specific miRNAs which may play critical roles in the conversion between NFs and CAFs in colorectal cancer (CRC) tissues, and will explain the mechanism of Combined Kushen Injection in the treatment of colorectal cancer. In this study, we will observe the effect of the inhibition of Combined Kushen Injection to CRC CAFs firstly. Then we will discover the critical miRNAs which influence the phenotype of CRC CAFs by comparing the miRNA expression profiles between NFs and CAFs and functional validation. Furthermore, we will either over-express or inhibit the candidate miRNAs in CAFs to observe whether the inhibition of Combined Kushen Injection to CAFs is mediated by the critical miRNAs. Finally, through comparing the mRNA expression profiles of NFs and CAFs and luciferase reporter assays, we will discuss possible mechanisms of the candidate miRNAs, and also the mechanisms of inhibition roles of Combined Kushen Injection to CAFs. The completion of this work will provide novel therapeutic target of colorectal cancer and explain the mechanism of Combined Kushen Injection in the treatment of colorectal cancer.