近年来研究显示可变剪切在肿瘤的发生发展中发挥了重要作用。我们前期发现剪切基因hnRNP A1在口腔癌中表达增高，促进了口腔癌细胞的生长，但其作用机制尚不清楚。进一步我们在口腔癌细胞中发现hnRNP A1的表达降低可以使CDK2 Isoform1的表达也随之降低，而CDK2 Isoform2的表达变化趋势相反。基于此，我们认为hnRNP A1可能通过选择性剪切CDK2而调控口腔癌的发生发展。本研究拟：通过进一步证明hnRNP A1对CDK2的调控作用，分析CDK2 Isoform1和CDK2 Isoform2在口腔癌中对肿瘤细胞生长的作用及其分子机制，对肿瘤的早期诊断及其治疗提供新的靶点。

Recent researches have revealed alternative splicing is critical in the development of tumor. Our previous research reported hnRNP A1 is critical in oral cancer and contributed to oral cancer progression. But its specific mechanism is still unknown. Furthermore, we revealed that the knockdown of hnRNP A1 could restrain the expression of CDK2 Isoform1, but promote the expression of CDK2 Isoform2. Thus, we speculated that hnRNP A1 could alternatively splice the expression of CDK2 to regulate the progression of oral cancer. Taking into account of previous researches and our study results, here, we try to explore the expression of hnRNP A1 and CDK2 in oral cancer and the corresponding adjacent tissues, and analyze the relationship and specific signalling pathway between hnRNP A1-CDK2 and oral cancer progression, which can be clinically used in early diagnosis and potential therapeutic target against oral cancer development.