三叉神经痛（TGN）发作时疼痛剧烈难忍，且易复发，虽然临床采用多种方法进行治疗，但效果很不满意，主要是对于其病因及病理生理机理尚不完全清楚。已有研究表明三磷酸腺苷（ATP）通过P2X受体亚型介导参与TGN致病过程，但是目前国内外缺乏这方面的系统研究。本研究在建立大鼠TGN模型的基础上，采用全细胞膜片钳等方法观察三叉神经节细胞膜何种P2X受体亚型表达量发生改变、三叉神经节细胞P2X受体电流特性是否发生改变以及细胞内钙信号变化特性是否发生改变等，从而系统研究和分析何种P2X受体亚型在TGN发生过程中起主要作用及其作用的可能细胞内信号转导途径，同时检测TGN患者相关神经节ATP受体表达和神经节细胞内P2X受体电流特性及细胞内信号转导途径变化规律；为深入了解ATP与TGN的关系及ATP在三叉神经痛的发生发展中扮演的角色及其作用机制，将对临床三叉神经痛治疗和药物开发提供一定的理论基础。

Trigeminal neuralgia (TGN) is one of refractory medical problem for a long time because the severe pain of it is difficult to endure during onset and easy relapse, and there has trigger points in face. Although a variety of methods for treatment had used in clinical practice, all of the therapeutic effect are not satisfied. Mainly reason is that the etiological and pathophysiological mechanisms are not entirely clear. Studies have shown that P2X receptor subtypes of adenosine triphosphate (ATP) are involved in the pathogenic process of TGN. However the systems research of it is lack of at present. Here, the TGN model rats and related ganglion (pterygopalotine ganglion, auriculotemporal ganglion and submandibular ganglion) of clinical patients will be studied in the possible mechanism of the variance of the P2X receptors dynamic expression, P2X receptor current, intracellular signal transduction, intracellular calcium and the axoplasmic transport of axis. Thus the systematic study and analysis which kinds of P2X subtype receptors will play a main role in the TGN process and its signal transduction pathway. The behavior detection will test the effectiveness of pharmacological intervention. From those design, the relationship between the ATP and TGN, and the mechanism of ATP in TGN development will be verified in thoroughly. Those will provide a basic theory for drug discovery to remedy radical trigeminal neuralgia treatment.